000249927 001__ 249927
000249927 005__ 20240118154005.0
000249927 037__ $$aDZNE-2023-00242
000249927 1001_ $$0P:(DE-2719)2810938$$aMüller, Stephan A$$b0$$eFirst author$$udzne
000249927 245__ $$aDataset: Secretome Analysis of hippocampal and cortical murine neurons
000249927 260__ $$bPRoteomics IDEntifications Database$$c2020
000249927 3367_ $$2BibTeX$$aMISC
000249927 3367_ $$0PUB:(DE-HGF)32$$2PUB:(DE-HGF)$$aDataset$$bdataset$$mdataset$$s1705588782_9225
000249927 3367_ $$026$$2EndNote$$aChart or Table
000249927 3367_ $$2DataCite$$aDataset
000249927 3367_ $$2ORCID$$aDATA_SET
000249927 3367_ $$2DINI$$aResearchData
000249927 520__ $$aTo understand how cells communicate with each other, it is essential to define the cellular secretome, a collection of proteins including soluble secreted, unconventionally secreted and proteolytically-shed proteins. Quantitative methodologies to decipher the secretome are challenging, due to the requirement of large cell numbers and abundant serum proteins that interfere with the detection of low-abundant cellular secretome proteins. Here, we have use the highe perfomance secretome-protein-enrichment-with-click-sugars method (hiSPECS) for glyco-secretome analysis. We applied this method to investigate differences of hippocampal and cortical murine neurons. Additionally, we have inhibited the Alzheimer related protease BACE1 to identify potential substrates in the secretome of hippocampal neurons.
000249927 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000249927 7001_ $$0P:(DE-2719)2181459$$aLichtenthaler, Stefan$$b1$$eLast author$$udzne
000249927 7870_ $$0DZNE-2021-00213$$aTüshaus, Johanna et.al.$$dHoboken, NJ [u.a.] : Wiley, 2020$$iRelatedTo$$r$$tAn optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome.
000249927 8564_ $$uhttps://www.ebi.ac.uk/pride/archive/projects/PXD020503
000249927 909CO $$ooai:pub.dzne.de:249927$$pVDB
000249927 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810938$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000249927 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2181459$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000249927 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000249927 9141_ $$y2020
000249927 9201_ $$0I:(DE-2719)1110006$$kAG Lichtenthaler$$lNeuroproteomics$$x0
000249927 980__ $$adataset
000249927 980__ $$aVDB
000249927 980__ $$aI:(DE-2719)1110006
000249927 980__ $$aUNRESTRICTED