Dataset

DZNE-2023-00242

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Secretome Analysis of hippocampal and cortical murine neurons

Müller, S. A. (First author)DZNE* ; Lichtenthaler, S. (Last author)DZNE*

2020
PRoteomics IDEntifications Database

Abstract: To understand how cells communicate with each other, it is essential to define the cellular secretome, a collection of proteins including soluble secreted, unconventionally secreted and proteolytically-shed proteins. Quantitative methodologies to decipher the secretome are challenging, due to the requirement of large cell numbers and abundant serum proteins that interfere with the detection of low-abundant cellular secretome proteins. Here, we have use the highe perfomance secretome-protein-enrichment-with-click-sugars method (hiSPECS) for glyco-secretome analysis. We applied this method to investigate differences of hippocampal and cortical murine neurons. Additionally, we have inhibited the Alzheimer related protease BACE1 to identify potential substrates in the secretome of hippocampal neurons.

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Contributing Institute(s):

1. Neuroproteomics (AG Lichtenthaler)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2020

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Tüshaus, J. (First author)DZNE* ; Müller, S. A.DZNE* ; Kataka, E. S. ; Zaucha, J. ; Sebastian Monasor, L.DZNE* ; Su, M.DZNE* ; Güner, G.DZNE* ; Jocher, G.DZNE* ; Tahirovic, S.DZNE* ; Frishman, D. ; Simons, M.DZNE* ; Lichtenthaler, S. (Last author)DZNE*
An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome.
The EMBO journal 39(20), e105693 (2020) [10.15252/embj.2020105693]2020   Files  Fulltext by Pubmed Central BibTeX | EndNote: XML, Text | RIS

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