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@MISC{Mller:249927,
      author       = {Müller, Stephan A and Lichtenthaler, Stefan},
      title        = {{D}ataset: {S}ecretome {A}nalysis of hippocampal and
                      cortical murine neurons},
      publisher    = {PRoteomics IDEntifications Database},
      reportid     = {DZNE-2023-00242},
      year         = {2020},
      abstract     = {To understand how cells communicate with each other, it is
                      essential to define the cellular secretome, a collection of
                      proteins including soluble secreted, unconventionally
                      secreted and proteolytically-shed proteins. Quantitative
                      methodologies to decipher the secretome are challenging, due
                      to the requirement of large cell numbers and abundant serum
                      proteins that interfere with the detection of low-abundant
                      cellular secretome proteins. Here, we have use the highe
                      perfomance secretome-protein-enrichment-with-click-sugars
                      method (hiSPECS) for glyco-secretome analysis. We applied
                      this method to investigate differences of hippocampal and
                      cortical murine neurons. Additionally, we have inhibited the
                      Alzheimer related protease BACE1 to identify potential
                      substrates in the secretome of hippocampal neurons.},
      cin          = {AG Lichtenthaler},
      cid          = {I:(DE-2719)1110006},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)32},
      url          = {https://pub.dzne.de/record/249927},
}