TY - JOUR AU - Sansevrino, Roberto AU - Hoffmann, Christian AU - Milovanovic, Dragomir TI - Condensate biology of synaptic vesicle clusters. JO - Trends in neurosciences VL - 46 IS - 4 SN - 0378-5912 CY - Amsterdam [u.a.] PB - Elsevier Science M1 - DZNE-2023-00322 SP - 293-306 PY - 2023 N1 - CC BY-NC-ND AB - Neuronal communication crucially relies on exocytosis of neurotransmitters from synaptic vesicles (SVs) which are clustered at synapses. To ensure reliable neurotransmitter release, synapses need to maintain an adequate pool of SVs at all times. Decades of research have established that SVs are clustered by synapsin 1, an abundant SV-associated phosphoprotein. The classical view postulates that SVs are crosslinked in a scaffold of protein-protein interactions between synapsins and their binding partners. Recent studies have shown that synapsins cluster SVs via liquid-liquid phase separation (LLPS), thus providing a new framework for the organization of the synapse. We discuss the evidence for phase separation of SVs, emphasizing emerging questions related to its regulation, specificity, and reversibility. KW - Humans KW - Synaptic Vesicles: metabolism KW - Synapsins: metabolism KW - Synapses: metabolism KW - Synaptic Transmission: physiology KW - Biology KW - liquid–liquid phase separation (Other) KW - liquid–liquid phase separation (Other) KW - neurotransmission (Other) KW - phosphorylation (Other) KW - synapse (Other) KW - synapsins (Other) KW - α-synuclein (Other) KW - α-synuclein (Other) KW - Synapsins (NLM Chemicals) LB - PUB:(DE-HGF)16 C6 - pmid:36725404 DO - DOI:10.1016/j.tins.2023.01.001 UR - https://pub.dzne.de/record/256460 ER -