TY  - JOUR
AU  - Andres-Alonso, Maria
AU  - Borgmeyer, Maximilian
AU  - Mirzapourdelavar, Hadi
AU  - Lormann, Jakob
AU  - Klein, Kim
AU  - Schweizer, Michaela
AU  - Hoffmeister-Ullerich, Sabine
AU  - Oelschlegel, Anja Maria
AU  - Dityatev, Alexander
AU  - Kreutz, Michael R
TI  - Golgi satellites are essential for polysialylation of NCAM and expression of LTP at distal synapses.
JO  - Cell reports
VL  - 42
IS  - 7
SN  - 2211-1247
CY  - [New York, NY]
PB  - Elsevier
M1  - DZNE-2023-00770
SP  - 112692
PY  - 2023
AB  - The complex cytoarchitecture of neurons poses significant challenges for the maturation of synaptic membrane proteins. It is currently unclear whether locally secreted synaptic proteins bypass the Golgi or whether they traffic through Golgi satellites (GSs). Here, we create a transgenic GS reporter mouse line and show that GSs are widely distributed along dendrites and are capable of mature glycosylation, in particular sialylation. We find that polysialylation of locally secreted NCAM takes place at GSs. Accordingly, in mice lacking a component of trans-Golgi network-to-plasma membrane trafficking, we find fewer GSs and significantly reduced PSA-NCAM levels in distal dendrites of CA1 neurons that receive input from the temporoammonic pathway. Induction of long-term potentiation at those, but not more proximal, synapses is severely impaired. We conclude that GSs serve the need for local mature glycosylation of synaptic membrane proteins in distal dendrites and thereby contribute to rapid changes in synaptic strength.
KW  - Mice
KW  - Animals
KW  - Long-Term Potentiation: physiology
KW  - Synapses: metabolism
KW  - Neurons: metabolism
KW  - Dendrites: metabolism
KW  - Neural Cell Adhesion Molecules: metabolism
KW  - CP: Cell biology (Other)
KW  - CP: Neuroscience (Other)
KW  - Golgi satellites (Other)
KW  - NCAM (Other)
KW  - polysialylation (Other)
KW  - secretory trafficking (Other)
KW  - synaptic plasticity (Other)
KW  - Neural Cell Adhesion Molecules (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:37355986
DO  - DOI:10.1016/j.celrep.2023.112692
UR  - https://pub.dzne.de/record/259698
ER  -