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000263609 037__ $$aDZNE-2023-00831
000263609 041__ $$aEnglish
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000263609 1001_ $$0P:(DE-2719)9002115$$aLovotti, Marta$$b0$$udzne
000263609 245__ $$aMonitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation.
000263609 260__ $$aNew York, NY$$bSpringer US$$c2024
000263609 29510 $$aTissue-Resident Macrophages / Mass, Elvira (Editor) ; New York, NY : Springer US, 2024, Chapter 28 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-3436-3=978-1-0716-3437-0 ; doi:10.1007/978-1-0716-3437-0
000263609 300__ $$a407 - 429
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000263609 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1694701779_30856
000263609 4900_ $$aMethods in Molecular Biology$$v2713
000263609 520__ $$aInflammasomes are intracellular, multiprotein supercomplexes that mediate a post-translational inflammatory response to both pathogen and endogenous danger signals. They consist of a sensor, the adapter ASC, and the protease caspase 1 and, following their activation, lead to cl1β, as well as lytic cell death. Due to this potent inflammatory capacity, understanding inflammasome biology is important in many pathological conditions. It is increasingly clear that inflammasomes are particularly relevant in macrophages, which express a diverse range of inflammasome sensors. In these two chapters, we detail methods to isolate and differentiate human macrophages, murine bone marrow-derived macrophages, and murine microglia and stimulate the inflammasomes known to be expressed in macrophages, including the AIM2, NLRP3, NLRC4, NLRP1, and non-canonical inflammasomes. Furthermore, we describe the methodology required to measure the various results of inflammasome activation including ASC speck formation, monitoring lytic cell death and cytokine release, as well as caspase-1 activation.
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000263609 650_7 $$2Other$$aAIM2
000263609 650_7 $$2Other$$aASC
000263609 650_7 $$2Other$$aCaspase 1
000263609 650_7 $$2Other$$aGasdermin D
000263609 650_7 $$2Other$$aInflammasome
000263609 650_7 $$2Other$$aNLRC4
000263609 650_7 $$2Other$$aNLRP1
000263609 650_7 $$2Other$$aNLRP3
000263609 650_7 $$2NLM Chemicals$$aInflammasomes
000263609 650_7 $$0EC 3.4.22.36$$2NLM Chemicals$$aCaspase 1
000263609 650_2 $$2MeSH$$aHumans
000263609 650_2 $$2MeSH$$aAnimals
000263609 650_2 $$2MeSH$$aMice
000263609 650_2 $$2MeSH$$aMicroglia
000263609 650_2 $$2MeSH$$aInflammasomes
000263609 650_2 $$2MeSH$$aMacrophages
000263609 650_2 $$2MeSH$$aCaspase 1
000263609 650_2 $$2MeSH$$aCell Death
000263609 7001_ $$0P:(DE-2719)2810930$$aMangan, Matthew$$b1$$udzne
000263609 7001_ $$0P:(DE-2719)2811671$$aMcManus, Róisín M$$b2$$eFirst author$$udzne
000263609 7001_ $$0P:(DE-2719)9002209$$aShkarina, Kateryna$$b3$$eFirst author$$udzne
000263609 7001_ $$aVasconcelos, Matilde B$$b4
000263609 7001_ $$0P:(DE-2719)2000062$$aLatz, Eicke$$b5$$eLast author$$udzne
000263609 773__ $$a10.1007/978-1-0716-3437-0_28
000263609 7870_ $$0DZNE-2023-00832$$aLovotti, Marta et.al.$$dNew York, NY : Springer US, 2024$$iRelatedTo$$r$$tMonitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation.
000263609 8564_ $$uhttps://pub.dzne.de/record/263609/files/DZNE-2023-00831_Restricted.pdf
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000263609 9141_ $$y2024
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000263609 9201_ $$0I:(DE-2719)1013024$$kAG Latz$$lInnate Immunity in Neurodegeneration$$x0
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