Contribution to a book

DZNE-2023-00831

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation.

Lovotti, M.Extern* ; Mangan, M.Extern* ; McManus, R. M. (First author)DZNE* ; Shkarina, K. (First author)DZNE* ; Vasconcelos, M. B. ; Latz, E. (Last author)DZNE*

2024
Springer US New York, NY
ISBN: 978-1-0716-3436-3 (print), 978-1-0716-3437-0 (electronic)

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Please use a persistent id in citations: doi:10.1007/978-1-0716-3437-0_28

Abstract: Inflammasomes are intracellular, multiprotein supercomplexes that mediate a post-translational inflammatory response to both pathogen and endogenous danger signals. They consist of a sensor, the adapter ASC, and the protease caspase 1 and, following their activation, lead to cl1β, as well as lytic cell death. Due to this potent inflammatory capacity, understanding inflammasome biology is important in many pathological conditions. It is increasingly clear that inflammasomes are particularly relevant in macrophages, which express a diverse range of inflammasome sensors. In these two chapters, we detail methods to isolate and differentiate human macrophages, murine bone marrow-derived macrophages, and murine microglia and stimulate the inflammasomes known to be expressed in macrophages, including the AIM2, NLRP3, NLRC4, NLRP1, and non-canonical inflammasomes. Furthermore, we describe the methodology required to measure the various results of inflammasome activation including ASC speck formation, monitoring lytic cell death and cytokine release, as well as caspase-1 activation.

Keyword(s): Humans (MeSH) ; Animals (MeSH) ; Mice (MeSH) ; Microglia (MeSH) ; Inflammasomes (MeSH) ; Macrophages (MeSH) ; Caspase 1 (MeSH) ; Cell Death (MeSH) ; AIM2 ; ASC ; Caspase 1 ; Gasdermin D ; Inflammasome ; NLRC4 ; NLRP1 ; NLRP3 ; Inflammasomes ; Caspase 1

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Contributing Institute(s):

1. Innate Immunity in Neurodegeneration (AG Latz)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2024

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Database coverage:
; SCOPUS

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Contribution to a book Lovotti, M.Extern* ; Mangan, M.Extern* ; McManus, R. M. (First author)DZNE* ; Shkarina, K. (First author)DZNE* ; Vasconcelos, M. B. ; Latz, E. (Last author)DZNE*
Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation.
2024Tissue-Resident Macrophages / Mass, Elvira (Editor) ; New York, NY : Springer US, 2024, Chapter 29 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-3436-3=978-1-0716-3437-0 ; doi:10.1007/978-1-0716-3437-0 New York, NY : Springer US, Methods in Molecular Biology 2713, 431 - 451 (2024) [10.1007/978-1-0716-3437-0_29]2024   Files BibTeX | EndNote: XML, Text | RIS

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