TY  - CHAP
AU  - Lovotti, Marta
AU  - Mangan, Matthew
AU  - McManus, Róisín M
AU  - Shkarina, Kateryna
AU  - Vasconcelos, Matilde B
AU  - Latz, Eicke
TI  - Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation.
VL  - 2713
CY  - New York, NY
PB  - Springer US
M1  - DZNE-2023-00831
SN  - 978-1-0716-3436-3 (print)
T2  - Methods in Molecular Biology
SP  - 407 - 429
PY  - 2024
AB  - Inflammasomes are intracellular, multiprotein supercomplexes that mediate a post-translational inflammatory response to both pathogen and endogenous danger signals. They consist of a sensor, the adapter ASC, and the protease caspase 1 and, following their activation, lead to cl1β, as well as lytic cell death. Due to this potent inflammatory capacity, understanding inflammasome biology is important in many pathological conditions. It is increasingly clear that inflammasomes are particularly relevant in macrophages, which express a diverse range of inflammasome sensors. In these two chapters, we detail methods to isolate and differentiate human macrophages, murine bone marrow-derived macrophages, and murine microglia and stimulate the inflammasomes known to be expressed in macrophages, including the AIM2, NLRP3, NLRC4, NLRP1, and non-canonical inflammasomes. Furthermore, we describe the methodology required to measure the various results of inflammasome activation including ASC speck formation, monitoring lytic cell death and cytokine release, as well as caspase-1 activation.
KW  - Humans
KW  - Animals
KW  - Mice
KW  - Microglia
KW  - Inflammasomes
KW  - Macrophages
KW  - Caspase 1
KW  - Cell Death
KW  - AIM2 (Other)
KW  - ASC (Other)
KW  - Caspase 1 (Other)
KW  - Gasdermin D (Other)
KW  - Inflammasome (Other)
KW  - NLRC4 (Other)
KW  - NLRP1 (Other)
KW  - NLRP3 (Other)
KW  - Inflammasomes (NLM Chemicals)
KW  - Caspase 1 (NLM Chemicals)
LB  - PUB:(DE-HGF)7
C6  - pmid:37639139
DO  - DOI:10.1007/978-1-0716-3437-0_28
UR  - https://pub.dzne.de/record/263609
ER  -