% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@INBOOK{Lovotti:263609,
      author       = {Lovotti, Marta and Mangan, Matthew and McManus, Róisín M
                      and Shkarina, Kateryna and Vasconcelos, Matilde B and Latz,
                      Eicke},
      title        = {{M}onitoring of {I}nflammasome {A}ctivation of
                      {M}acrophages and {M}icroglia {I}n {V}itro, {P}art 1: {C}ell
                      {P}reparation and {I}nflammasome {S}timulation.},
      volume       = {2713},
      address      = {New York, NY},
      publisher    = {Springer US},
      reportid     = {DZNE-2023-00831},
      isbn         = {978-1-0716-3436-3 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {407 - 429},
      year         = {2024},
      comment      = {Tissue-Resident Macrophages / Mass, Elvira (Editor) ; New
                      York, NY : Springer US, 2024, Chapter 28 ; ISSN:
                      1064-3745=1940-6029 ; ISBN:
                      978-1-0716-3436-3=978-1-0716-3437-0 ;
                      doi:10.1007/978-1-0716-3437-0},
      booktitle     = {Tissue-Resident Macrophages / Mass,
                       Elvira (Editor) ; New York, NY :
                       Springer US, 2024, Chapter 28 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-0716-3436-3=978-1-0716-3437-0 ;
                       doi:10.1007/978-1-0716-3437-0},
      abstract     = {Inflammasomes are intracellular, multiprotein
                      supercomplexes that mediate a post-translational
                      inflammatory response to both pathogen and endogenous danger
                      signals. They consist of a sensor, the adapter ASC, and the
                      protease caspase 1 and, following their activation, lead to
                      cl1β, as well as lytic cell death. Due to this potent
                      inflammatory capacity, understanding inflammasome biology is
                      important in many pathological conditions. It is
                      increasingly clear that inflammasomes are particularly
                      relevant in macrophages, which express a diverse range of
                      inflammasome sensors. In these two chapters, we detail
                      methods to isolate and differentiate human macrophages,
                      murine bone marrow-derived macrophages, and murine microglia
                      and stimulate the inflammasomes known to be expressed in
                      macrophages, including the AIM2, NLRP3, NLRC4, NLRP1, and
                      non-canonical inflammasomes. Furthermore, we describe the
                      methodology required to measure the various results of
                      inflammasome activation including ASC speck formation,
                      monitoring lytic cell death and cytokine release, as well as
                      caspase-1 activation.},
      keywords     = {Humans / Animals / Mice / Microglia / Inflammasomes /
                      Macrophages / Caspase 1 / Cell Death / AIM2 (Other) / ASC
                      (Other) / Caspase 1 (Other) / Gasdermin D (Other) /
                      Inflammasome (Other) / NLRC4 (Other) / NLRP1 (Other) / NLRP3
                      (Other) / Inflammasomes (NLM Chemicals) / Caspase 1 (NLM
                      Chemicals)},
      cin          = {AG Latz},
      ddc          = {570},
      cid          = {I:(DE-2719)1013024},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)7},
      pubmed       = {pmid:37639139},
      doi          = {10.1007/978-1-0716-3437-0_28},
      url          = {https://pub.dzne.de/record/263609},
}