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| Home > Publications Database > Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation. > print |
| 001 | 263609 | ||
| 005 | 20240402133626.0 | ||
| 020 | _ | _ | |a 978-1-0716-3436-3 (print) |
| 020 | _ | _ | |a 978-1-0716-3437-0 (electronic) |
| 024 | 7 | _ | |a 10.1007/978-1-0716-3437-0_28 |2 doi |
| 024 | 7 | _ | |a pmid:37639139 |2 pmid |
| 024 | 7 | _ | |a 1064-3745 |2 ISSN |
| 024 | 7 | _ | |a 1940-6029 |2 ISSN |
| 024 | 7 | _ | |a altmetric:153416887 |2 altmetric |
| 037 | _ | _ | |a DZNE-2023-00831 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Lovotti, Marta |0 P:(DE-2719)9002115 |b 0 |u dzne |
| 245 | _ | _ | |a Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation. |
| 260 | _ | _ | |a New York, NY |c 2024 |b Springer US |
| 295 | 1 | 0 | |a Tissue-Resident Macrophages / Mass, Elvira (Editor) ; New York, NY : Springer US, 2024, Chapter 28 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-3436-3=978-1-0716-3437-0 ; doi:10.1007/978-1-0716-3437-0 |
| 300 | _ | _ | |a 407 - 429 |
| 336 | 7 | _ | |a BOOK_CHAPTER |2 ORCID |
| 336 | 7 | _ | |a Book Section |0 7 |2 EndNote |
| 336 | 7 | _ | |a bookPart |2 DRIVER |
| 336 | 7 | _ | |a INBOOK |2 BibTeX |
| 336 | 7 | _ | |a Output Types/Book chapter |2 DataCite |
| 336 | 7 | _ | |a Contribution to a book |b contb |m contb |0 PUB:(DE-HGF)7 |s 1694701779_30856 |2 PUB:(DE-HGF) |
| 490 | 0 | _ | |a Methods in Molecular Biology |v 2713 |
| 520 | _ | _ | |a Inflammasomes are intracellular, multiprotein supercomplexes that mediate a post-translational inflammatory response to both pathogen and endogenous danger signals. They consist of a sensor, the adapter ASC, and the protease caspase 1 and, following their activation, lead to cl1β, as well as lytic cell death. Due to this potent inflammatory capacity, understanding inflammasome biology is important in many pathological conditions. It is increasingly clear that inflammasomes are particularly relevant in macrophages, which express a diverse range of inflammasome sensors. In these two chapters, we detail methods to isolate and differentiate human macrophages, murine bone marrow-derived macrophages, and murine microglia and stimulate the inflammasomes known to be expressed in macrophages, including the AIM2, NLRP3, NLRC4, NLRP1, and non-canonical inflammasomes. Furthermore, we describe the methodology required to measure the various results of inflammasome activation including ASC speck formation, monitoring lytic cell death and cytokine release, as well as caspase-1 activation. |
| 536 | _ | _ | |a 351 - Brain Function (POF4-351) |0 G:(DE-HGF)POF4-351 |c POF4-351 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de |
| 650 | _ | 7 | |a AIM2 |2 Other |
| 650 | _ | 7 | |a ASC |2 Other |
| 650 | _ | 7 | |a Caspase 1 |2 Other |
| 650 | _ | 7 | |a Gasdermin D |2 Other |
| 650 | _ | 7 | |a Inflammasome |2 Other |
| 650 | _ | 7 | |a NLRC4 |2 Other |
| 650 | _ | 7 | |a NLRP1 |2 Other |
| 650 | _ | 7 | |a NLRP3 |2 Other |
| 650 | _ | 7 | |a Inflammasomes |2 NLM Chemicals |
| 650 | _ | 7 | |a Caspase 1 |0 EC 3.4.22.36 |2 NLM Chemicals |
| 650 | _ | 2 | |a Humans |2 MeSH |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Mice |2 MeSH |
| 650 | _ | 2 | |a Microglia |2 MeSH |
| 650 | _ | 2 | |a Inflammasomes |2 MeSH |
| 650 | _ | 2 | |a Macrophages |2 MeSH |
| 650 | _ | 2 | |a Caspase 1 |2 MeSH |
| 650 | _ | 2 | |a Cell Death |2 MeSH |
| 700 | 1 | _ | |a Mangan, Matthew |0 P:(DE-2719)2810930 |b 1 |u dzne |
| 700 | 1 | _ | |a McManus, Róisín M |0 P:(DE-2719)2811671 |b 2 |e First author |u dzne |
| 700 | 1 | _ | |a Shkarina, Kateryna |0 P:(DE-2719)9002209 |b 3 |e First author |u dzne |
| 700 | 1 | _ | |a Vasconcelos, Matilde B |b 4 |
| 700 | 1 | _ | |a Latz, Eicke |0 P:(DE-2719)2000062 |b 5 |e Last author |u dzne |
| 773 | _ | _ | |a 10.1007/978-1-0716-3437-0_28 |
| 787 | 0 | _ | |a Lovotti, Marta et.al. |d New York, NY : Springer US, 2024 |i RelatedTo |0 DZNE-2023-00832 |r |t Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation. |
| 856 | 4 | _ | |u https://pub.dzne.de/record/263609/files/DZNE-2023-00831_Restricted.pdf |
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| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Neurodegenerative Diseases |1 G:(DE-HGF)POF4-350 |0 G:(DE-HGF)POF4-351 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-300 |4 G:(DE-HGF)POF |v Brain Function |x 0 |
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| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2022-11-23 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2022-11-23 |
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