TY  - CHAP
AU  - Lovotti, Marta
AU  - Mangan, Matthew
AU  - McManus, Róisín M
AU  - Shkarina, Kateryna
AU  - Vasconcelos, Matilde B
AU  - Latz, Eicke
TI  - Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation.
VL  - 2713
CY  - New York, NY
PB  - Springer US
M1  - DZNE-2023-00832
SN  - 978-1-0716-3436-3 (print)
T2  - Methods in Molecular Biology
SP  - 431 - 451
PY  - 2024
AB  - Inflammasomes are macromolecular complexes that assemble upon the detection of cytoplasmic pathogen-associated or danger-associated signals and induce a necrotic type of cell death termed pyroptosis, facilitating pro-inflammatory cytokine release. Inflammasomes play a critical role in innate immunity and inflammatory response; however, they have also been associated with multiple diseases, including autoinflammatory and neurodegenerative conditions. In the following chapter, we describe methods to detect inflammasome activation and its downstream effects, including detection of ASC oligomerization, detection of activated caspase-1 and cleaved IL-1β, as well as read-outs for inflammasome-mediated cell death.
KW  - Microglia
KW  - Inflammasomes
KW  - Macrophages
KW  - Immunity, Innate
KW  - Caspase 1
KW  - ASC speck (Other)
KW  - Cytokine production (Other)
KW  - Inflammasome (Other)
KW  - LDH release (Other)
KW  - Pyroptosis (Other)
KW  - Western blot (Other)
KW  - Inflammasomes (NLM Chemicals)
KW  - Caspase 1 (NLM Chemicals)
LB  - PUB:(DE-HGF)7
C6  - pmid:37639140
DO  - DOI:10.1007/978-1-0716-3437-0_29
UR  - https://pub.dzne.de/record/263610
ER  -