Contribution to a book

DZNE-2023-00832

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation.

Lovotti, M.Extern* ; Mangan, M.Extern* ; McManus, R. M. (First author)DZNE* ; Shkarina, K. (First author)DZNE* ; Vasconcelos, M. B. ; Latz, E. (Last author)DZNE*

2024
Springer US New York, NY
ISBN: 978-1-0716-3436-3 (print), 978-1-0716-3437-0 (electronic)

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Please use a persistent id in citations: doi:10.1007/978-1-0716-3437-0_29

Abstract: Inflammasomes are macromolecular complexes that assemble upon the detection of cytoplasmic pathogen-associated or danger-associated signals and induce a necrotic type of cell death termed pyroptosis, facilitating pro-inflammatory cytokine release. Inflammasomes play a critical role in innate immunity and inflammatory response; however, they have also been associated with multiple diseases, including autoinflammatory and neurodegenerative conditions. In the following chapter, we describe methods to detect inflammasome activation and its downstream effects, including detection of ASC oligomerization, detection of activated caspase-1 and cleaved IL-1β, as well as read-outs for inflammasome-mediated cell death.

Keyword(s): Microglia (MeSH) ; Inflammasomes (MeSH) ; Macrophages (MeSH) ; Immunity, Innate (MeSH) ; Caspase 1 (MeSH) ; ASC speck ; Cytokine production ; Inflammasome ; LDH release ; Pyroptosis ; Western blot ; Inflammasomes ; Caspase 1

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Contributing Institute(s):

1. Innate Immunity in Neurodegeneration (AG Latz)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2024

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Database coverage:
; SCOPUS

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Contribution to a book Lovotti, M.Extern* ; Mangan, M.Extern* ; McManus, R. M. (First author)DZNE* ; Shkarina, K. (First author)DZNE* ; Vasconcelos, M. B. ; Latz, E. (Last author)DZNE*
Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation.
2024Tissue-Resident Macrophages / Mass, Elvira (Editor) ; New York, NY : Springer US, 2024, Chapter 28 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-3436-3=978-1-0716-3437-0 ; doi:10.1007/978-1-0716-3437-0 New York, NY : Springer US, Methods in Molecular Biology 2713, 407 - 429 (2024) [10.1007/978-1-0716-3437-0_28]2024   Files BibTeX | EndNote: XML, Text | RIS

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