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@INBOOK{Lovotti:263610,
author = {Lovotti, Marta and Mangan, Matthew and McManus, Róisín M
and Shkarina, Kateryna and Vasconcelos, Matilde B and Latz,
Eicke},
title = {{M}onitoring of {I}nflammasome {A}ctivation of
{M}acrophages and {M}icroglia {I}n {V}itro, {P}art 2:
{A}ssessing {I}nflammasome {A}ctivation.},
volume = {2713},
address = {New York, NY},
publisher = {Springer US},
reportid = {DZNE-2023-00832},
isbn = {978-1-0716-3436-3 (print)},
series = {Methods in Molecular Biology},
pages = {431 - 451},
year = {2024},
comment = {Tissue-Resident Macrophages / Mass, Elvira (Editor) ; New
York, NY : Springer US, 2024, Chapter 29 ; ISSN:
1064-3745=1940-6029 ; ISBN:
978-1-0716-3436-3=978-1-0716-3437-0 ;
doi:10.1007/978-1-0716-3437-0},
booktitle = {Tissue-Resident Macrophages / Mass,
Elvira (Editor) ; New York, NY :
Springer US, 2024, Chapter 29 ; ISSN:
1064-3745=1940-6029 ; ISBN:
978-1-0716-3436-3=978-1-0716-3437-0 ;
doi:10.1007/978-1-0716-3437-0},
abstract = {Inflammasomes are macromolecular complexes that assemble
upon the detection of cytoplasmic pathogen-associated or
danger-associated signals and induce a necrotic type of cell
death termed pyroptosis, facilitating pro-inflammatory
cytokine release. Inflammasomes play a critical role in
innate immunity and inflammatory response; however, they
have also been associated with multiple diseases, including
autoinflammatory and neurodegenerative conditions. In the
following chapter, we describe methods to detect
inflammasome activation and its downstream effects,
including detection of ASC oligomerization, detection of
activated caspase-1 and cleaved IL-1β, as well as read-outs
for inflammasome-mediated cell death.},
keywords = {Microglia / Inflammasomes / Macrophages / Immunity, Innate
/ Caspase 1 / ASC speck (Other) / Cytokine production
(Other) / Inflammasome (Other) / LDH release (Other) /
Pyroptosis (Other) / Western blot (Other) / Inflammasomes
(NLM Chemicals) / Caspase 1 (NLM Chemicals)},
cin = {AG Latz},
ddc = {570},
cid = {I:(DE-2719)1013024},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)7},
pubmed = {pmid:37639140},
doi = {10.1007/978-1-0716-3437-0_29},
url = {https://pub.dzne.de/record/263610},
}