001     263610
005     20240402133626.0
020 _ _ |a 978-1-0716-3436-3 (print)
020 _ _ |a 978-1-0716-3437-0 (electronic)
024 7 _ |a 10.1007/978-1-0716-3437-0_29
|2 doi
024 7 _ |a pmid:37639140
|2 pmid
024 7 _ |a 1064-3745
|2 ISSN
024 7 _ |a 1940-6029
|2 ISSN
024 7 _ |a altmetric:153416888
|2 altmetric
037 _ _ |a DZNE-2023-00832
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Lovotti, Marta
|0 P:(DE-2719)9002115
|b 0
|u dzne
245 _ _ |a Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation.
260 _ _ |a New York, NY
|c 2024
|b Springer US
295 1 0 |a Tissue-Resident Macrophages / Mass, Elvira (Editor) ; New York, NY : Springer US, 2024, Chapter 29 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-3436-3=978-1-0716-3437-0 ; doi:10.1007/978-1-0716-3437-0
300 _ _ |a 431 - 451
336 7 _ |a BOOK_CHAPTER
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336 7 _ |a INBOOK
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336 7 _ |a Output Types/Book chapter
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336 7 _ |a Contribution to a book
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|m contb
|0 PUB:(DE-HGF)7
|s 1694701678_30860
|2 PUB:(DE-HGF)
490 0 _ |a Methods in Molecular Biology
|v 2713
520 _ _ |a Inflammasomes are macromolecular complexes that assemble upon the detection of cytoplasmic pathogen-associated or danger-associated signals and induce a necrotic type of cell death termed pyroptosis, facilitating pro-inflammatory cytokine release. Inflammasomes play a critical role in innate immunity and inflammatory response; however, they have also been associated with multiple diseases, including autoinflammatory and neurodegenerative conditions. In the following chapter, we describe methods to detect inflammasome activation and its downstream effects, including detection of ASC oligomerization, detection of activated caspase-1 and cleaved IL-1β, as well as read-outs for inflammasome-mediated cell death.
536 _ _ |a 351 - Brain Function (POF4-351)
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|c POF4-351
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de
650 _ 7 |a ASC speck
|2 Other
650 _ 7 |a Cytokine production
|2 Other
650 _ 7 |a Inflammasome
|2 Other
650 _ 7 |a LDH release
|2 Other
650 _ 7 |a Pyroptosis
|2 Other
650 _ 7 |a Western blot
|2 Other
650 _ 7 |a Inflammasomes
|2 NLM Chemicals
650 _ 7 |a Caspase 1
|0 EC 3.4.22.36
|2 NLM Chemicals
650 _ 2 |a Microglia
|2 MeSH
650 _ 2 |a Inflammasomes
|2 MeSH
650 _ 2 |a Macrophages
|2 MeSH
650 _ 2 |a Immunity, Innate
|2 MeSH
650 _ 2 |a Caspase 1
|2 MeSH
700 1 _ |a Mangan, Matthew
|0 P:(DE-2719)2810930
|b 1
|u dzne
700 1 _ |a McManus, Róisín M
|0 P:(DE-2719)2811671
|b 2
|e First author
|u dzne
700 1 _ |a Shkarina, Kateryna
|0 P:(DE-2719)9002209
|b 3
|e First author
|u dzne
700 1 _ |a Vasconcelos, Matilde B
|b 4
700 1 _ |a Latz, Eicke
|0 P:(DE-2719)2000062
|b 5
|e Last author
|u dzne
773 _ _ |a 10.1007/978-1-0716-3437-0_29
856 4 _ |u https://pub.dzne.de/record/263610/files/DZNE-2023-00832.pdf
856 4 _ |u https://pub.dzne.de/record/263610/files/DZNE-2023-00832.pdf?subformat=pdfa
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909 C O |o oai:pub.dzne.de:263610
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910 1 _ |a External Institute
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910 1 _ |a External Institute
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
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|6 P:(DE-2719)2811671
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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913 1 _ |a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|1 G:(DE-HGF)POF4-350
|0 G:(DE-HGF)POF4-351
|3 G:(DE-HGF)POF4
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|v Brain Function
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914 1 _ |y 2024
915 _ _ |a DBCoverage
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|d 2022-11-23
915 _ _ |a DBCoverage
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920 1 _ |0 I:(DE-2719)1013024
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|x 0
980 _ _ |a contb
980 _ _ |a VDB
980 _ _ |a I:(DE-2719)1013024
980 _ _ |a UNRESTRICTED


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