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000263633 005__ 20240724152745.0
000263633 0247_ $$2doi$$a10.5281/ZENODO.6990849
000263633 037__ $$aDZNE-2023-00851
000263633 1001_ $$0P:(DE-2719)2810270$$aBradke, Frank$$b0$$udzne
000263633 245__ $$aSoftware: Microtubule retrograde flow retains neuronal polarization in a fluctuating state (v1)
000263633 260__ $$aZenodo$$c2022
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000263633 520__ $$aIn developing vertebrate neurons, a neurite is formed by more than a hundred microtubules. While individual microtubules are dynamic, the microtubule array has been regarded as stationary. Using live-cell imaging of neurons in culture or in brain slices, combined with photoconversion techniques and pharmacological manipulations, we uncovered that the microtubule array flows retrogradely within neurites to the soma. This flow drives cycles of microtubule density, a hallmark of the fluctuating state before axon formation, thereby inhibiting neurite growth. The motor protein dynein fuels this process. Shortly after axon formation, microtubule retrograde flow slows down in the axon, reducing microtubule density cycles and enabling axon extension. Thus, keeping neurites short is an active process. Microtubule retrograde flow is a novel type of cytoskeletal dynamics, which changes the hitherto axon-centric view of neuronal polarization.
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000263633 650_7 $$2Other$$aNeurons
000263633 650_7 $$2Other$$acell biology
000263633 650_7 $$2Other$$acytoskeleton
000263633 650_7 $$2Other$$amicrotubules
000263633 650_7 $$2Other$$aLive cell imaging
000263633 650_7 $$2Other$$aPython
000263633 650_7 $$2Other$$aautomated analysis
000263633 650_7 $$2Other$$amicroscopy
000263633 650_7 $$2Other$$aDynein
000263633 650_7 $$2Other$$afigureflow
000263633 7001_ $$0P:(DE-2719)2811316$$aSchelski, Max$$b1$$udzne
000263633 773__ $$a10.5281/ZENODO.6990849
000263633 7870_ $$0DZNE-2022-01732$$aSchelski, Max et.al.$$dWashington, DC [u.a.] : Assoc., 2022$$iRelatedTo$$tMicrotubule retrograde flow retains neuronal polarization in a fluctuating state.
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000263633 9141_ $$y2022
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000263633 9201_ $$0I:(DE-2719)1013002$$kAG Bradke$$lAxon Growth and Regeneration$$x0
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