Software

DZNE-2023-00851

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Software: Microtubule retrograde flow retains neuronal polarization in a fluctuating state (v1)

Bradke, F.DZNE* ; Schelski, M.DZNE*

2022
Zenodo

This record in other databases:  

Please use a persistent id in citations: doi:10.5281/ZENODO.6990849

Abstract: In developing vertebrate neurons, a neurite is formed by more than a hundred microtubules. While individual microtubules are dynamic, the microtubule array has been regarded as stationary. Using live-cell imaging of neurons in culture or in brain slices, combined with photoconversion techniques and pharmacological manipulations, we uncovered that the microtubule array flows retrogradely within neurites to the soma. This flow drives cycles of microtubule density, a hallmark of the fluctuating state before axon formation, thereby inhibiting neurite growth. The motor protein dynein fuels this process. Shortly after axon formation, microtubule retrograde flow slows down in the axon, reducing microtubule density cycles and enabling axon extension. Thus, keeping neurites short is an active process. Microtubule retrograde flow is a novel type of cytoskeletal dynamics, which changes the hitherto axon-centric view of neuronal polarization.

Keyword(s): Neurons ; cell biology ; cytoskeleton ; microtubules ; Live cell imaging ; Python ; automated analysis ; microscopy ; Dynein ; figureflow

---

Contributing Institute(s):

1. Axon Growth and Regeneration (AG Bradke)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2022

---

---

Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Software Bradke, F. (First author)DZNE* ; Schelski, M. (Last author)DZNE*
Software: Microtubule retrograde flow retains neuronal polarization in a fluctuating state, v2
Zenodo (2022) [10.5281/ZENODO.7120626]2022 BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Schelski, M. (First author)DZNE* ; Bradke, F. (Last author)DZNE*
Microtubule retrograde flow retains neuronal polarization in a fluctuating state.
Science advances 8(44), eabo2336 (2022) [10.1126/sciadv.abo2336]2022   Files  Fulltext Fulltext by Pubmed Central BibTeX | EndNote: XML, Text | RIS

---