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001     263633
005     20240724152745.0
024 7 _ |2 doi
|a 10.5281/ZENODO.6990849
037 _ _ |a DZNE-2023-00851
100 1 _ |0 P:(DE-2719)2810270
|a Bradke, Frank
|b 0
|u dzne
245 _ _ |a Software: Microtubule retrograde flow retains neuronal polarization in a fluctuating state (v1)
260 _ _ |a Zenodo
|c 2022
336 7 _ |2 DCMI
|a Software
336 7 _ |0 PUB:(DE-HGF)33
|2 PUB:(DE-HGF)
|a Software
|b sware
|m sware
|s 1721827560_7926
336 7 _ |2 BibTeX
|a MISC
336 7 _ |0 6
|2 EndNote
|a Computer Program
336 7 _ |2 ORCID
|a OTHER
336 7 _ |2 DataCite
|a Software
520 _ _ |a In developing vertebrate neurons, a neurite is formed by more than a hundred microtubules. While individual microtubules are dynamic, the microtubule array has been regarded as stationary. Using live-cell imaging of neurons in culture or in brain slices, combined with photoconversion techniques and pharmacological manipulations, we uncovered that the microtubule array flows retrogradely within neurites to the soma. This flow drives cycles of microtubule density, a hallmark of the fluctuating state before axon formation, thereby inhibiting neurite growth. The motor protein dynein fuels this process. Shortly after axon formation, microtubule retrograde flow slows down in the axon, reducing microtubule density cycles and enabling axon extension. Thus, keeping neurites short is an active process. Microtubule retrograde flow is a novel type of cytoskeletal dynamics, which changes the hitherto axon-centric view of neuronal polarization.
536 _ _ |0 G:(DE-HGF)POF4-351
|a 351 - Brain Function (POF4-351)
|c POF4-351
|f POF IV
|x 0
588 _ _ |a Dataset connected to DataCite
650 _ 7 |2 Other
|a Neurons
650 _ 7 |2 Other
|a cell biology
650 _ 7 |2 Other
|a cytoskeleton
650 _ 7 |2 Other
|a microtubules
650 _ 7 |2 Other
|a Live cell imaging
650 _ 7 |2 Other
|a Python
650 _ 7 |2 Other
|a automated analysis
650 _ 7 |2 Other
|a microscopy
650 _ 7 |2 Other
|a Dynein
650 _ 7 |2 Other
|a figureflow
700 1 _ |0 P:(DE-2719)2811316
|a Schelski, Max
|b 1
|u dzne
773 _ _ |a 10.5281/ZENODO.6990849
787 0 _ |0 DZNE-2022-01732
|a Schelski, Max et.al.
|d Washington, DC [u.a.] : Assoc., 2022
|i RelatedTo
|t Microtubule retrograde flow retains neuronal polarization in a fluctuating state.
909 C O |o oai:pub.dzne.de:263633
|p VDB
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)2810270
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 0
|k DZNE
910 1 _ |0 I:(DE-588)1065079516
|6 P:(DE-2719)2811316
|a Deutsches Zentrum für Neurodegenerative Erkrankungen
|b 1
|k DZNE
913 1 _ |0 G:(DE-HGF)POF4-351
|1 G:(DE-HGF)POF4-350
|2 G:(DE-HGF)POF4-300
|3 G:(DE-HGF)POF4
|4 G:(DE-HGF)POF
|a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|v Brain Function
|x 0
914 1 _ |y 2022
920 1 _ |0 I:(DE-2719)1013002
|k AG Bradke
|l Axon Growth and Regeneration
|x 0
980 _ _ |a sware
980 _ _ |a VDB
980 _ _ |a I:(DE-2719)1013002
980 _ _ |a UNRESTRICTED

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