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000266486 0247_ $$2doi$$a10.1016/j.xpro.2023.102559
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000266486 037__ $$aDZNE-2023-01171
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000266486 1001_ $$0P:(DE-2719)2812671$$aAntignano, Ignazio$$b0$$eFirst author$$udzne
000266486 245__ $$aAssessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages
000266486 260__ $$aAmsterdam$$bElsevier$$c2023
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000266486 520__ $$aProtein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins. Here, we present an optimized protocol for assessing the translation rate in mouse adult microglia and cultured bone-marrow-derived macrophages. We describe steps for isolating cells, treating them with a puromycin-analog probe, and fluorescently labeling the puromycylated-polypeptide chains. We then detail their quantification by flow cytometry or with a fluorescent plate reader. For complete details on the use and execution of this protocol, please refer to Keane et al. (2021).1.
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000266486 650_2 $$2MeSH$$aAnimals
000266486 650_2 $$2MeSH$$aMice
000266486 650_2 $$2MeSH$$aMicroglia
000266486 650_2 $$2MeSH$$aBone Marrow
000266486 650_2 $$2MeSH$$aMacrophages
000266486 650_2 $$2MeSH$$aColoring Agents
000266486 650_2 $$2MeSH$$aProtein Biosynthesis: genetics
000266486 650_7 $$2NLM Chemicals$$aColoring Agents
000266486 650_7 $$2Other$$aFlow Cytometry/Mass Cytometry
000266486 650_7 $$2Other$$aImmunology
000266486 650_7 $$2Other$$aNeuroscience
000266486 7001_ $$0P:(DE-2719)2812184$$aKeane, Lily$$b1$$udzne
000266486 7001_ $$0P:(DE-2719)2811780$$aCapasso, Melania$$b2$$eLast author
000266486 773__ $$0PERI:(DE-600)3053335-1$$a10.1016/j.xpro.2023.102559$$gVol. 4, no. 4, p. 102559 -$$n4$$p102559$$tSTAR Protocols$$v4$$x2666-1667$$y2023
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000266486 9141_ $$y2023
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