Journal Article

DZNE-2023-01171

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages

Antignano, I. (First author)DZNE* ; Keane, L.DZNE* ; Capasso, M. (Last author)DZNE*

2023
Elsevier Amsterdam

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Please use a persistent id in citations: doi:10.1016/j.xpro.2023.102559

Abstract: Protein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins. Here, we present an optimized protocol for assessing the translation rate in mouse adult microglia and cultured bone-marrow-derived macrophages. We describe steps for isolating cells, treating them with a puromycin-analog probe, and fluorescently labeling the puromycylated-polypeptide chains. We then detail their quantification by flow cytometry or with a fluorescent plate reader. For complete details on the use and execution of this protocol, please refer to Keane et al. (2021).1.

Keyword(s): Animals (MeSH) ; Mice (MeSH) ; Microglia (MeSH) ; Bone Marrow (MeSH) ; Macrophages (MeSH) ; Coloring Agents (MeSH) ; Protein Biosynthesis: genetics (MeSH) ; Coloring Agents ; Flow Cytometry/Mass Cytometry ; Immunology ; Neuroscience

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Contributing Institute(s):

1. Immune Regulation (AG Capasso)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2023

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Database coverage:
; ; ; ; Article Processing Charges ; DOAJ Seal ; Fees ; PubMed Central ; SCOPUS

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