TY  - JOUR
AU  - Antignano, Ignazio
AU  - Keane, Lily
AU  - Capasso, Melania
TI  - Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages
JO  - STAR Protocols
VL  - 4
IS  - 4
SN  - 2666-1667
CY  - Amsterdam
PB  - Elsevier
M1  - DZNE-2023-01171
SP  - 102559
PY  - 2023
AB  - Protein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins. Here, we present an optimized protocol for assessing the translation rate in mouse adult microglia and cultured bone-marrow-derived macrophages. We describe steps for isolating cells, treating them with a puromycin-analog probe, and fluorescently labeling the puromycylated-polypeptide chains. We then detail their quantification by flow cytometry or with a fluorescent plate reader. For complete details on the use and execution of this protocol, please refer to Keane et al. (2021).1.
KW  - Animals
KW  - Mice
KW  - Microglia
KW  - Bone Marrow
KW  - Macrophages
KW  - Coloring Agents
KW  - Protein Biosynthesis: genetics
KW  - Coloring Agents (NLM Chemicals)
KW  - Flow Cytometry/Mass Cytometry (Other)
KW  - Immunology (Other)
KW  - Neuroscience (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37713309
C2  - pmc:PMC10509706
DO  - DOI:10.1016/j.xpro.2023.102559
UR  - https://pub.dzne.de/record/266486
ER  -