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@ARTICLE{Antignano:266486,
      author       = {Antignano, Ignazio and Keane, Lily and Capasso, Melania},
      title        = {{A}ssessing m{RNA} translation in mouse adult microglia and
                      bone-marrow-derived macrophages},
      journal      = {STAR Protocols},
      volume       = {4},
      number       = {4},
      issn         = {2666-1667},
      address      = {Amsterdam},
      publisher    = {Elsevier},
      reportid     = {DZNE-2023-01171},
      pages        = {102559},
      year         = {2023},
      abstract     = {Protein synthesis, or mRNA translation, is the biological
                      process through which genetic information stored in
                      messenger RNAs is encoded into proteins. Here, we present an
                      optimized protocol for assessing the translation rate in
                      mouse adult microglia and cultured bone-marrow-derived
                      macrophages. We describe steps for isolating cells, treating
                      them with a puromycin-analog probe, and fluorescently
                      labeling the puromycylated-polypeptide chains. We then
                      detail their quantification by flow cytometry or with a
                      fluorescent plate reader. For complete details on the use
                      and execution of this protocol, please refer to Keane et al.
                      (2021).1.},
      keywords     = {Animals / Mice / Microglia / Bone Marrow / Macrophages /
                      Coloring Agents / Protein Biosynthesis: genetics / Coloring
                      Agents (NLM Chemicals) / Flow Cytometry/Mass Cytometry
                      (Other) / Immunology (Other) / Neuroscience (Other)},
      cin          = {AG Capasso},
      ddc          = {600},
      cid          = {I:(DE-2719)1013033},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37713309},
      pmc          = {pmc:PMC10509706},
      doi          = {10.1016/j.xpro.2023.102559},
      url          = {https://pub.dzne.de/record/266486},
}