Home > Publications Database > Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages > print

001     266486
005     20240403115010.0
024 7 _ |a pmid:37713309
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024 7 _ |a 10.1016/j.xpro.2023.102559
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024 7 _ |a pmc:PMC10509706
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037 _ _ |a DZNE-2023-01171
041 _ _ |a English
082 _ _ |a 600
100 1 _ |a Antignano, Ignazio
|0 P:(DE-2719)2812671
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245 _ _ |a Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages
260 _ _ |a Amsterdam
|c 2023
|b Elsevier
336 7 _ |a article
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336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
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336 7 _ |a JOURNAL_ARTICLE
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336 7 _ |a Journal Article
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520 _ _ |a Protein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins. Here, we present an optimized protocol for assessing the translation rate in mouse adult microglia and cultured bone-marrow-derived macrophages. We describe steps for isolating cells, treating them with a puromycin-analog probe, and fluorescently labeling the puromycylated-polypeptide chains. We then detail their quantification by flow cytometry or with a fluorescent plate reader. For complete details on the use and execution of this protocol, please refer to Keane et al. (2021).1.
536 _ _ |a 351 - Brain Function (POF4-351)
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588 _ _ |a Dataset connected to CrossRef, Journals: pub.dzne.de
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Microglia
|2 MeSH
650 _ 2 |a Bone Marrow
|2 MeSH
650 _ 2 |a Macrophages
|2 MeSH
650 _ 2 |a Coloring Agents
|2 MeSH
650 _ 2 |a Protein Biosynthesis: genetics
|2 MeSH
650 _ 7 |a Coloring Agents
|2 NLM Chemicals
650 _ 7 |a Flow Cytometry/Mass Cytometry
|2 Other
650 _ 7 |a Immunology
|2 Other
650 _ 7 |a Neuroscience
|2 Other
700 1 _ |a Keane, Lily
|0 P:(DE-2719)2812184
|b 1
|u dzne
700 1 _ |a Capasso, Melania
|0 P:(DE-2719)2811780
|b 2
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773 _ _ |a 10.1016/j.xpro.2023.102559
|g Vol. 4, no. 4, p. 102559 -
|0 PERI:(DE-600)3053335-1
|n 4
|p 102559
|t STAR Protocols
|v 4
|y 2023
|x 2666-1667
856 4 _ |y OpenAccess
|u https://pub.dzne.de/record/266486/files/DZNE-2023-01171.pdf
856 4 _ |y OpenAccess
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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913 1 _ |a DE-HGF
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914 1 _ |y 2023
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