TY  - JOUR
AU  - Methi, Aditi
AU  - Islam, Rezaul
AU  - Kaurani, Lalit
AU  - Sakib, M Sadman
AU  - Krüger, Dennis M
AU  - Pena, Tonatiuh
AU  - Burkhardt, Susanne
AU  - Liebetanz, David
AU  - Fischer, André
TI  - A Single-Cell Transcriptomic Analysis of the Mouse Hippocampus After Voluntary Exercise.
JO  - Molecular neurobiology
VL  - 61
IS  - 8
SN  - 0893-7648
CY  - Totowa, NJ
PB  - Humana Press
M1  - DZNE-2024-00879
SP  - 5628 - 5645
PY  - 2024
AB  - Exercise has been recognized as a beneficial factor for cognitive health, particularly in relation to the hippocampus, a vital brain region responsible for learning and memory. Previous research has demonstrated that exercise-mediated improvement of learning and memory in humans and rodents correlates with increased adult neurogenesis and processes related to enhanced synaptic plasticity. Nevertheless, the underlying molecular mechanisms are not fully understood. With the aim to further elucidate these mechanisms, we provide a comprehensive dataset of the mouse hippocampal transcriptome at the single-cell level after 4 weeks of voluntary wheel-running. Our analysis provides a number of interesting observations. For example, the results suggest that exercise affects adult neurogenesis by accelerating the maturation of a subpopulation of Prdm16-expressing neurons. Moreover, we uncover the existence of an intricate crosstalk among multiple vital signaling pathways such as NF-κB, Wnt/β-catenin, Notch, and retinoic acid (RA) pathways altered upon exercise in a specific cluster of excitatory neurons within the Cornu Ammonis (CA) region of the hippocampus. In conclusion, our study provides an important resource dataset and sheds further light on the molecular changes induced by exercise in the hippocampus. These findings have implications for developing targeted interventions aimed at optimizing cognitive health and preventing age-related cognitive decline.
KW  - Animals
KW  - Hippocampus: metabolism
KW  - Physical Conditioning, Animal: physiology
KW  - Single-Cell Analysis
KW  - Gene Expression Profiling
KW  - Transcriptome: genetics
KW  - Mice, Inbred C57BL
KW  - Mice
KW  - Male
KW  - Neurogenesis
KW  - Neurons: metabolism
KW  - Signal Transduction
KW  - Volition
KW  - Aerobic exercise (Other)
KW  - Cognitive decline (Other)
KW  - Dementia (Other)
KW  - Environmental enrichment (Other)
KW  - Gene-expression (Other)
KW  - Hippocampus (Other)
KW  - Learning and memory (Other)
KW  - Single-cell RNAseq (Other)
LB  - PUB:(DE-HGF)16
C2  - pmc:PMC11249425
C6  - pmid:38217668
DO  - DOI:10.1007/s12035-023-03869-9
UR  - https://pub.dzne.de/record/270707
ER  -