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000275837 041__ $$aEnglish
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000275837 1001_ $$00000-0001-5222-4987$$aColonna, Marco$$b0
000275837 245__ $$aImplementation and validation of single-cell genomics experiments in neuroscience.
000275837 260__ $$aNew York, NY$$bNature America$$c2024
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000275837 520__ $$aSingle-cell or single-nucleus transcriptomics is a powerful tool for identifying cell types and cell states. However, hypotheses derived from these assays, including gene expression information, require validation, and their functional relevance needs to be established. The choice of validation depends on numerous factors. Here, we present types of orthogonal and functional validation experiment to strengthen preliminary findings obtained using single-cell and single-nucleus transcriptomics as well as the challenges and limitations of these approaches.
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000275837 650_2 $$2MeSH$$aSingle-Cell Analysis: methods
000275837 650_2 $$2MeSH$$aNeurosciences: methods
000275837 650_2 $$2MeSH$$aAnimals
000275837 650_2 $$2MeSH$$aGenomics: methods
000275837 650_2 $$2MeSH$$aHumans
000275837 650_2 $$2MeSH$$aTranscriptome
000275837 650_2 $$2MeSH$$aGene Expression Profiling: methods
000275837 7001_ $$00000-0002-3363-7302$$aKonopka, Genevieve$$b1
000275837 7001_ $$00000-0002-0840-1437$$aLiddelow, Shane A$$b2
000275837 7001_ $$00000-0003-2345-4964$$aNowakowski, Tomasz$$b3
000275837 7001_ $$00000-0002-0713-2140$$aAwatramani, Rajeshwar$$b4
000275837 7001_ $$00000-0002-0135-0972$$aBateup, Helen S$$b5
000275837 7001_ $$00000-0003-1963-8285$$aCadwell, Cathryn R$$b6
000275837 7001_ $$00000-0001-5340-8614$$aCaglayan, Emre$$b7
000275837 7001_ $$00000-0002-7409-7859$$aChen, Jerry L$$b8
000275837 7001_ $$00000-0002-0936-9774$$aGillis, Jesse$$b9
000275837 7001_ $$00000-0002-3819-7019$$aKampmann, Martin$$b10
000275837 7001_ $$00000-0002-1400-6820$$aKrienen, Fenna$$b11
000275837 7001_ $$00000-0002-3012-6945$$aMarsh, Samuel E$$b12
000275837 7001_ $$00000-0002-3547-237X$$aMonje, Michelle$$b13
000275837 7001_ $$00000-0002-6135-7607$$aO'Dea, Michael R$$b14
000275837 7001_ $$00000-0002-3825-7675$$aPatani, Rickie$$b15
000275837 7001_ $$00000-0003-3263-8634$$aPollen, Alex A$$b16
000275837 7001_ $$00000-0001-8156-0736$$aQuintana, Francisco J$$b17
000275837 7001_ $$aScavuzzo, Marissa$$b18
000275837 7001_ $$aSchmitz, Matthew$$b19
000275837 7001_ $$00000-0001-7769-7684$$aSloan, Steven A$$b20
000275837 7001_ $$00000-0003-1532-3155$$aTesar, Paul J$$b21
000275837 7001_ $$00000-0001-5991-7620$$aTollkuhn, Jessica$$b22
000275837 7001_ $$00000-0001-6338-9357$$aTosches, Maria Antonietta$$b23
000275837 7001_ $$aUrbanek, Madeleine E$$b24
000275837 7001_ $$00000-0001-9248-6420$$aWerner, Jonathan M$$b25
000275837 7001_ $$00000-0001-6055-277X$$aBayraktar, Omer A$$b26
000275837 7001_ $$0P:(DE-2719)9002754$$aGokce, Ozgun$$b27
000275837 7001_ $$00000-0002-6049-2487$$aHabib, Naomi$$b28
000275837 773__ $$0PERI:(DE-600)1494955-6$$a10.1038/s41593-024-01814-0$$gVol. 27, no. 12, p. 2310 - 2325$$n12$$p2310 - 2325$$tNature neuroscience$$v27$$x1097-6256$$y2024
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