Journal Article (Review Article)

DZNE-2025-00072

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Implementation and validation of single-cell genomics experiments in neuroscience.

Colonna, M. ; Konopka, G. ; Liddelow, S. A. ; Nowakowski, T. ; Awatramani, R. ; Bateup, H. S. ; Cadwell, C. R. ; Caglayan, E. ; Chen, J. L. ; Gillis, J. ; Kampmann, M. ; Krienen, F. ; Marsh, S. E. ; Monje, M. ; O'Dea, M. R. ; Patani, R. ; Pollen, A. A. ; Quintana, F. J. ; Scavuzzo, M. ; Schmitz, M. ; Sloan, S. A. ; Tesar, P. J. ; Tollkuhn, J. ; Tosches, M. A. ; Urbanek, M. E. ; Werner, J. M. ; Bayraktar, O. A. ; Gokce, O.DZNE* ; Habib, N.

2024
Nature America New York, NY

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Please use a persistent id in citations: doi:10.1038/s41593-024-01814-0

Abstract: Single-cell or single-nucleus transcriptomics is a powerful tool for identifying cell types and cell states. However, hypotheses derived from these assays, including gene expression information, require validation, and their functional relevance needs to be established. The choice of validation depends on numerous factors. Here, we present types of orthogonal and functional validation experiment to strengthen preliminary findings obtained using single-cell and single-nucleus transcriptomics as well as the challenges and limitations of these approaches.

Keyword(s): Single-Cell Analysis: methods (MeSH) ; Neurosciences: methods (MeSH) ; Animals (MeSH) ; Genomics: methods (MeSH) ; Humans (MeSH) ; Transcriptome (MeSH) ; Gene Expression Profiling: methods (MeSH)

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Contributing Institute(s):

1. Spatial Dynamics of Neurodegeneration (AG Gokce)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Nature ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 25 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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