TY  - JOUR
AU  - Colonna, Marco
AU  - Konopka, Genevieve
AU  - Liddelow, Shane A
AU  - Nowakowski, Tomasz
AU  - Awatramani, Rajeshwar
AU  - Bateup, Helen S
AU  - Cadwell, Cathryn R
AU  - Caglayan, Emre
AU  - Chen, Jerry L
AU  - Gillis, Jesse
AU  - Kampmann, Martin
AU  - Krienen, Fenna
AU  - Marsh, Samuel E
AU  - Monje, Michelle
AU  - O'Dea, Michael R
AU  - Patani, Rickie
AU  - Pollen, Alex A
AU  - Quintana, Francisco J
AU  - Scavuzzo, Marissa
AU  - Schmitz, Matthew
AU  - Sloan, Steven A
AU  - Tesar, Paul J
AU  - Tollkuhn, Jessica
AU  - Tosches, Maria Antonietta
AU  - Urbanek, Madeleine E
AU  - Werner, Jonathan M
AU  - Bayraktar, Omer A
AU  - Gokce, Ozgun
AU  - Habib, Naomi
TI  - Implementation and validation of single-cell genomics experiments in neuroscience.
JO  - Nature neuroscience
VL  - 27
IS  - 12
SN  - 1097-6256
CY  - New York, NY
PB  - Nature America
M1  - DZNE-2025-00072
SP  - 2310 - 2325
PY  - 2024
AB  - Single-cell or single-nucleus transcriptomics is a powerful tool for identifying cell types and cell states. However, hypotheses derived from these assays, including gene expression information, require validation, and their functional relevance needs to be established. The choice of validation depends on numerous factors. Here, we present types of orthogonal and functional validation experiment to strengthen preliminary findings obtained using single-cell and single-nucleus transcriptomics as well as the challenges and limitations of these approaches.
KW  - Single-Cell Analysis: methods
KW  - Neurosciences: methods
KW  - Animals
KW  - Genomics: methods
KW  - Humans
KW  - Transcriptome
KW  - Gene Expression Profiling: methods
LB  - PUB:(DE-HGF)16
C6  - pmid:39627589
DO  - DOI:10.1038/s41593-024-01814-0
UR  - https://pub.dzne.de/record/275837
ER  -