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001     275837
005     20250119000338.0
024 7 _ |a 10.1038/s41593-024-01814-0
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024 7 _ |a 1097-6256
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024 7 _ |a 1546-1726
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041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Colonna, Marco
|0 0000-0001-5222-4987
|b 0
245 _ _ |a Implementation and validation of single-cell genomics experiments in neuroscience.
260 _ _ |a New York, NY
|c 2024
|b Nature America
336 7 _ |a article
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520 _ _ |a Single-cell or single-nucleus transcriptomics is a powerful tool for identifying cell types and cell states. However, hypotheses derived from these assays, including gene expression information, require validation, and their functional relevance needs to be established. The choice of validation depends on numerous factors. Here, we present types of orthogonal and functional validation experiment to strengthen preliminary findings obtained using single-cell and single-nucleus transcriptomics as well as the challenges and limitations of these approaches.
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650 _ 2 |a Single-Cell Analysis: methods
|2 MeSH
650 _ 2 |a Neurosciences: methods
|2 MeSH
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Genomics: methods
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Transcriptome
|2 MeSH
650 _ 2 |a Gene Expression Profiling: methods
|2 MeSH
700 1 _ |a Konopka, Genevieve
|0 0000-0002-3363-7302
|b 1
700 1 _ |a Liddelow, Shane A
|0 0000-0002-0840-1437
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700 1 _ |a Nowakowski, Tomasz
|0 0000-0003-2345-4964
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700 1 _ |a Awatramani, Rajeshwar
|0 0000-0002-0713-2140
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700 1 _ |a Bateup, Helen S
|0 0000-0002-0135-0972
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700 1 _ |a Cadwell, Cathryn R
|0 0000-0003-1963-8285
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700 1 _ |a Caglayan, Emre
|0 0000-0001-5340-8614
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700 1 _ |a Chen, Jerry L
|0 0000-0002-7409-7859
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700 1 _ |a Gillis, Jesse
|0 0000-0002-0936-9774
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700 1 _ |a Kampmann, Martin
|0 0000-0002-3819-7019
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700 1 _ |a Krienen, Fenna
|0 0000-0002-1400-6820
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700 1 _ |a Marsh, Samuel E
|0 0000-0002-3012-6945
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700 1 _ |a Monje, Michelle
|0 0000-0002-3547-237X
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700 1 _ |a O'Dea, Michael R
|0 0000-0002-6135-7607
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700 1 _ |a Patani, Rickie
|0 0000-0002-3825-7675
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700 1 _ |a Pollen, Alex A
|0 0000-0003-3263-8634
|b 16
700 1 _ |a Quintana, Francisco J
|0 0000-0001-8156-0736
|b 17
700 1 _ |a Scavuzzo, Marissa
|b 18
700 1 _ |a Schmitz, Matthew
|b 19
700 1 _ |a Sloan, Steven A
|0 0000-0001-7769-7684
|b 20
700 1 _ |a Tesar, Paul J
|0 0000-0003-1532-3155
|b 21
700 1 _ |a Tollkuhn, Jessica
|0 0000-0001-5991-7620
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700 1 _ |a Tosches, Maria Antonietta
|0 0000-0001-6338-9357
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700 1 _ |a Urbanek, Madeleine E
|b 24
700 1 _ |a Werner, Jonathan M
|0 0000-0001-9248-6420
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700 1 _ |a Bayraktar, Omer A
|0 0000-0001-6055-277X
|b 26
700 1 _ |a Gokce, Ozgun
|0 P:(DE-2719)9002754
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700 1 _ |a Habib, Naomi
|0 0000-0002-6049-2487
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773 _ _ |a 10.1038/s41593-024-01814-0
|g Vol. 27, no. 12, p. 2310 - 2325
|0 PERI:(DE-600)1494955-6
|n 12
|p 2310 - 2325
|t Nature neuroscience
|v 27
|y 2024
|x 1097-6256
856 4 _ |u https://pub.dzne.de/record/275837/files/DZNE-2025-00072_Restricted.pdf
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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Marc 21