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001     275837
005     20260212170206.0
024 7 _ |2 doi
|a 10.1038/s41593-024-01814-0
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|a pmid:39627589
024 7 _ |2 ISSN
|a 1097-6256
024 7 _ |2 ISSN
|a 1546-1726
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|a altmetric:171492556
037 _ _ |a DZNE-2025-00072
041 _ _ |a English
082 _ _ |a 610
100 1 _ |0 0000-0001-5222-4987
|a Colonna, Marco
|b 0
245 _ _ |a Implementation and validation of single-cell genomics experiments in neuroscience.
260 _ _ |a New York, NY
|b Nature America
|c 2024
336 7 _ |2 DRIVER
|a article
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|a Journal Article
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|s 1736775085_27320
|x Review Article
336 7 _ |2 BibTeX
|a ARTICLE
336 7 _ |2 ORCID
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|a Journal Article
520 _ _ |a Single-cell or single-nucleus transcriptomics is a powerful tool for identifying cell types and cell states. However, hypotheses derived from these assays, including gene expression information, require validation, and their functional relevance needs to be established. The choice of validation depends on numerous factors. Here, we present types of orthogonal and functional validation experiment to strengthen preliminary findings obtained using single-cell and single-nucleus transcriptomics as well as the challenges and limitations of these approaches.
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650 _ 2 |2 MeSH
|a Single-Cell Analysis: methods
650 _ 2 |2 MeSH
|a Neurosciences: methods
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Genomics: methods
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Transcriptome
650 _ 2 |2 MeSH
|a Gene Expression Profiling: methods
700 1 _ |0 0000-0002-3363-7302
|a Konopka, Genevieve
|b 1
700 1 _ |0 0000-0002-0840-1437
|a Liddelow, Shane A
|b 2
700 1 _ |0 0000-0003-2345-4964
|a Nowakowski, Tomasz
|b 3
700 1 _ |0 0000-0002-0713-2140
|a Awatramani, Rajeshwar
|b 4
700 1 _ |0 0000-0002-0135-0972
|a Bateup, Helen S
|b 5
700 1 _ |0 0000-0003-1963-8285
|a Cadwell, Cathryn R
|b 6
700 1 _ |0 0000-0001-5340-8614
|a Caglayan, Emre
|b 7
700 1 _ |0 0000-0002-7409-7859
|a Chen, Jerry L
|b 8
700 1 _ |0 0000-0002-0936-9774
|a Gillis, Jesse
|b 9
700 1 _ |0 0000-0002-3819-7019
|a Kampmann, Martin
|b 10
700 1 _ |0 0000-0002-1400-6820
|a Krienen, Fenna
|b 11
700 1 _ |0 0000-0002-3012-6945
|a Marsh, Samuel E
|b 12
700 1 _ |0 0000-0002-3547-237X
|a Monje, Michelle
|b 13
700 1 _ |0 0000-0002-6135-7607
|a O'Dea, Michael R
|b 14
700 1 _ |0 0000-0002-3825-7675
|a Patani, Rickie
|b 15
700 1 _ |0 0000-0003-3263-8634
|a Pollen, Alex A
|b 16
700 1 _ |0 0000-0001-8156-0736
|a Quintana, Francisco J
|b 17
700 1 _ |a Scavuzzo, Marissa
|b 18
700 1 _ |a Schmitz, Matthew
|b 19
700 1 _ |0 0000-0001-7769-7684
|a Sloan, Steven A
|b 20
700 1 _ |0 0000-0003-1532-3155
|a Tesar, Paul J
|b 21
700 1 _ |0 0000-0001-5991-7620
|a Tollkuhn, Jessica
|b 22
700 1 _ |0 0000-0001-6338-9357
|a Tosches, Maria Antonietta
|b 23
700 1 _ |a Urbanek, Madeleine E
|b 24
700 1 _ |0 0000-0001-9248-6420
|a Werner, Jonathan M
|b 25
700 1 _ |0 0000-0001-6055-277X
|a Bayraktar, Omer A
|b 26
700 1 _ |0 P:(DE-2719)9002754
|a Gokce, Ozgun
|b 27
700 1 _ |0 0000-0002-6049-2487
|a Habib, Naomi
|b 28
773 _ _ |0 PERI:(DE-600)1494955-6
|a 10.1038/s41593-024-01814-0
|g Vol. 27, no. 12, p. 2310 - 2325
|n 12
|p 2310 - 2325
|t Nature neuroscience
|v 27
|x 1097-6256
|y 2024
856 4 _ |u https://pub.dzne.de/record/275837/files/DZNE-2025-00072_Restricted.pdf
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LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21