TY  - JOUR
AU  - Eckstein, Yasmin
AU  - Kessler, Barbara
AU  - Hinrichs, Arne
AU  - Novak, Istvan
AU  - von Thaden, Anne
AU  - Lorenzen, Timo
AU  - Rathkolb, Birgit
AU  - Scholz, Armin
AU  - Blutke, Andreas
AU  - Koopmans, Sietse-Jan
AU  - de Angelis, Martin Hrabĕ
AU  - Christoffersen, Berit Østergaard
AU  - Wolf, Eckhard
AU  - Renner, Simone
TI  - Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.
JO  - STAR Protocols
VL  - 6
IS  - 2
SN  - 2666-1667
CY  - Cambridge, MA
PB  - Cell Press
M1  - DZNE-2025-00547
SP  - 103774
PY  - 2025
AB  - The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2.
KW  - Animals
KW  - Swine
KW  - Insulin Secretion: physiology
KW  - Insulin: metabolism
KW  - Insulin: blood
KW  - Blood Glucose: metabolism
KW  - Blood Glucose: analysis
KW  - Glucose Clamp Technique: methods
KW  - Glucagon: blood
KW  - C-Peptide: blood
KW  - Glucose Tolerance Test: methods
KW  - Biotechnology and bioengineering (Other)
KW  - Metabolism (Other)
KW  - Model Organisms (Other)
LB  - PUB:(DE-HGF)16
C2  - pmc:PMC12224880
C6  - pmid:40249709
DO  - DOI:10.1016/j.xpro.2025.103774
UR  - https://pub.dzne.de/record/278041
ER  -