Journal Article

DZNE-2025-00547

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.

Eckstein, Y. ; Kessler, B. ; Hinrichs, A. ; Novak, I. ; von Thaden, A.DZNE* ; Lorenzen, T. ; Rathkolb, B. ; Scholz, A. ; Blutke, A. ; Koopmans, S.-J. ; de Angelis, M. H. ; Christoffersen, B. Ø. ; Wolf, E. ; Renner, S.

2025
Cell Press Cambridge, MA

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Please use a persistent id in citations: doi:10.1016/j.xpro.2025.103774

Abstract: The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2.

Keyword(s): Animals (MeSH) ; Swine (MeSH) ; Insulin Secretion: physiology (MeSH) ; Insulin: metabolism (MeSH) ; Insulin: blood (MeSH) ; Blood Glucose: metabolism (MeSH) ; Blood Glucose: analysis (MeSH) ; Glucose Clamp Technique: methods (MeSH) ; Glucagon: blood (MeSH) ; C-Peptide: blood (MeSH) ; Glucose Tolerance Test: methods (MeSH) ; Biotechnology and bioengineering ; Metabolism ; Model Organisms

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Contributing Institute(s):

1. Mouse Facility (Munich) (Mouse Facility (Munich))

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2025

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