%0 Journal Article
%A Fuhrmann, Falko
%A Nebeling, Felix C
%A Musacchio, Fabrizio
%A Mittag, Manuel
%A Poll, Stefanie
%A Müller, Monika
%A Ambrad Giovannetti, Eleonora
%A Maibach, Michael
%A Schaffran, Barbara
%A Burnside, Emily
%A Chan, Chi Wai
%A Lagurin, Alex Simon
%A Reichenbach, Nicole
%A Kaushalya, Sanjeev Kumar
%A Fried, Hans
%A Linden, Stefan
%A Petzold, Gabor C
%A Tavosanis, Gaia
%A Bradke, Frank
%A Fuhrmann, Martin
%T Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.
%J Communications biology
%V 8
%N 1
%@ 2399-3642
%C London
%I Springer Nature
%M DZNE-2025-00647
%P 795
%D 2025
%X The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ± 0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes' motility (58.9 ± 2
%K Animals
%K Prefrontal Cortex: cytology
%K Prefrontal Cortex: diagnostic imaging
%K Prefrontal Cortex: physiology
%K Neurons: physiology
%K Neurons: cytology
%K Neurons: metabolism
%K Mice
%K Neuroglia: cytology
%K Neuroglia: physiology
%K Neuroglia: metabolism
%K Male
%K Microscopy, Fluorescence, Multiphoton: methods
%K Mice, Inbred C57BL
%K Dendritic Spines
%K Calcium: metabolism
%K Calcium (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40410447
%2 pmc:PMC12102176
%R 10.1038/s42003-025-08079-8
%U https://pub.dzne.de/record/278921