Journal Article

DZNE-2025-00647

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.

Fuhrmann, F. (First author)DZNE* ; Nebeling, F. C. (First author)DZNE* ; Musacchio, F. (First author)DZNE* ; Mittag, M.DZNE* ; Poll, S.DZNE* ; Müller, M.DZNE* ; Ambrad Giovannetti, E.DZNE* ; Maibach, M.DZNE* ; Schaffran, B.DZNE* ; Burnside, E.DZNE* ; Chan, C. W.DZNE* ; Lagurin, A. S.DZNE* ; Reichenbach, N.DZNE* ; Kaushalya, S. K.DZNE* ; Fried, H.DZNE* ; Linden, S. ; Petzold, G. C.DZNE* ; Tavosanis, G.DZNE* ; Bradke, F.DZNE* ; Fuhrmann, M. (Last author)DZNE*

2025
Springer Nature London

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Please use a persistent id in citations: doi:10.1038/s42003-025-08079-8

Abstract: The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ± 0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes' motility (58.9 ± 2% turnover rate) at previously unreachable depths (1100 µm). We establish three-photon imaging of the mPFC enabling neuronal and glial recordings with subcellular resolution that will pave the way for novel discoveries in this brain region.

Keyword(s): Animals (MeSH) ; Prefrontal Cortex: cytology (MeSH) ; Prefrontal Cortex: diagnostic imaging (MeSH) ; Prefrontal Cortex: physiology (MeSH) ; Neurons: physiology (MeSH) ; Neurons: cytology (MeSH) ; Neurons: metabolism (MeSH) ; Mice (MeSH) ; Neuroglia: cytology (MeSH) ; Neuroglia: physiology (MeSH) ; Neuroglia: metabolism (MeSH) ; Male (MeSH) ; Microscopy, Fluorescence, Multiphoton: methods (MeSH) ; Mice, Inbred C57BL (MeSH) ; Dendritic Spines (MeSH) ; Calcium: metabolism (MeSH) ; Calcium

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Contributing Institute(s):

1. Neuroimmunology and Imaging (AG Fuhrmann)
2. Axon Growth and Regeneration (AG Bradke)
3. Vascular Neurology (AG Petzold)
4. Dynamics of neuronal circuits (AG Tavosanis)
5. Light Microscopy Facility (CRFS-LMF) (LMF)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)
2. 351 - Brain Function (POF4-351) (POF4-351)
3. 353 - Clinical and Health Care Research (POF4-353) (POF4-353)
4. 899 - ohne Topic (POF4-899) (POF4-899)

Experiment(s):

1. Light Microscope Facility (CRFS-LMF) / Bonn

Appears in the scientific report 2025

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Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset Fuhrmann, F.DZNE* ; Nebeling, F.DZNE* ; Musacchio, F.DZNE* ; Mittag, M.DZNE* ; Poll, S.Extern* ; Müller, M.DZNE* ; Ambrad Giovannetti, E.DZNE* ; Maibach, M.DZNE* ; Schaffran, B.DZNE* ; Burnside, E.DZNE* ; Chan, C. W. ; Lagurin, A. S.DZNE* ; Reichenbach, N.DZNE* ; Kaushalya, S. ; Fried, H.DZNE* ; Linden, S. ; Petzold, G.DZNE* ; Tavosanis, G.Extern* ; Bradke, F.DZNE* ; Fuhrmann, M.DZNE*
Dataset: Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution
Dryad (2025) [10.5061/dryad.tqjq2bw90]2025 BibTeX | EndNote: XML, Text | RIS

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