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000278921 1001_ $$0P:(DE-2719)2811225$$aFuhrmann, Falko$$b0$$eFirst author$$udzne
000278921 245__ $$aThree-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.
000278921 260__ $$aLondon$$bSpringer Nature$$c2025
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000278921 520__ $$aThe medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ± 0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes' motility (58.9 ± 2% turnover rate) at previously unreachable depths (1100 µm). We establish three-photon imaging of the mPFC enabling neuronal and glial recordings with subcellular resolution that will pave the way for novel discoveries in this brain region.
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000278921 650_7 $$0SY7Q814VUP$$2NLM Chemicals$$aCalcium
000278921 650_2 $$2MeSH$$aAnimals
000278921 650_2 $$2MeSH$$aPrefrontal Cortex: cytology
000278921 650_2 $$2MeSH$$aPrefrontal Cortex: diagnostic imaging
000278921 650_2 $$2MeSH$$aPrefrontal Cortex: physiology
000278921 650_2 $$2MeSH$$aNeurons: physiology
000278921 650_2 $$2MeSH$$aNeurons: cytology
000278921 650_2 $$2MeSH$$aNeurons: metabolism
000278921 650_2 $$2MeSH$$aMice
000278921 650_2 $$2MeSH$$aNeuroglia: cytology
000278921 650_2 $$2MeSH$$aNeuroglia: physiology
000278921 650_2 $$2MeSH$$aNeuroglia: metabolism
000278921 650_2 $$2MeSH$$aMale
000278921 650_2 $$2MeSH$$aMicroscopy, Fluorescence, Multiphoton: methods
000278921 650_2 $$2MeSH$$aMice, Inbred C57BL
000278921 650_2 $$2MeSH$$aDendritic Spines
000278921 650_2 $$2MeSH$$aCalcium: metabolism
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000278921 7001_ $$0P:(DE-2719)2811414$$aNebeling, Felix C$$b1$$eFirst author$$udzne
000278921 7001_ $$0P:(DE-2719)2812689$$aMusacchio, Fabrizio$$b2$$eFirst author$$udzne
000278921 7001_ $$0P:(DE-2719)2811044$$aMittag, Manuel$$b3$$udzne
000278921 7001_ $$0P:(DE-2719)2810397$$aPoll, Stefanie$$b4
000278921 7001_ $$0P:(DE-2719)2811495$$aMüller, Monika$$b5
000278921 7001_ $$0P:(DE-2719)2811489$$aAmbrad Giovannetti, Eleonora$$b6$$udzne
000278921 7001_ $$0P:(DE-HGF)0$$aMaibach, Michael$$b7
000278921 7001_ $$0P:(DE-2719)2811123$$aSchaffran, Barbara$$b8$$udzne
000278921 7001_ $$0P:(DE-2719)9000509$$aBurnside, Emily$$b9$$udzne
000278921 7001_ $$0P:(DE-2719)9001354$$aChan, Chi Wai$$b10
000278921 7001_ $$0P:(DE-HGF)0$$aLagurin, Alex Simon$$b11
000278921 7001_ $$0P:(DE-2719)2811281$$aReichenbach, Nicole$$b12$$udzne
000278921 7001_ $$0P:(DE-2719)2812833$$aKaushalya, Sanjeev Kumar$$b13
000278921 7001_ $$0P:(DE-2719)2191568$$aFried, Hans$$b14$$udzne
000278921 7001_ $$aLinden, Stefan$$b15
000278921 7001_ $$0P:(DE-2719)2810273$$aPetzold, Gabor C$$b16
000278921 7001_ $$0P:(DE-2719)2810271$$aTavosanis, Gaia$$b17
000278921 7001_ $$0P:(DE-2719)2810270$$aBradke, Frank$$b18
000278921 7001_ $$0P:(DE-2719)2679991$$aFuhrmann, Martin$$b19$$eLast author
000278921 773__ $$0PERI:(DE-600)2919698-X$$a10.1038/s42003-025-08079-8$$gVol. 8, no. 1, p. 795$$n1$$p795$$tCommunications biology$$v8$$x2399-3642$$y2025
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