TY  - JOUR
AU  - Fuhrmann, Falko
AU  - Nebeling, Felix C
AU  - Musacchio, Fabrizio
AU  - Mittag, Manuel
AU  - Poll, Stefanie
AU  - Müller, Monika
AU  - Ambrad Giovannetti, Eleonora
AU  - Maibach, Michael
AU  - Schaffran, Barbara
AU  - Burnside, Emily
AU  - Chan, Chi Wai
AU  - Lagurin, Alex Simon
AU  - Reichenbach, Nicole
AU  - Kaushalya, Sanjeev Kumar
AU  - Fried, Hans
AU  - Linden, Stefan
AU  - Petzold, Gabor C
AU  - Tavosanis, Gaia
AU  - Bradke, Frank
AU  - Fuhrmann, Martin
TI  - Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.
JO  - Communications biology
VL  - 8
IS  - 1
SN  - 2399-3642
CY  - London
PB  - Springer Nature
M1  - DZNE-2025-00647
SP  - 795
PY  - 2025
AB  - The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ± 0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes' motility (58.9 ± 2
KW  - Animals
KW  - Prefrontal Cortex: cytology
KW  - Prefrontal Cortex: diagnostic imaging
KW  - Prefrontal Cortex: physiology
KW  - Neurons: physiology
KW  - Neurons: cytology
KW  - Neurons: metabolism
KW  - Mice
KW  - Neuroglia: cytology
KW  - Neuroglia: physiology
KW  - Neuroglia: metabolism
KW  - Male
KW  - Microscopy, Fluorescence, Multiphoton: methods
KW  - Mice, Inbred C57BL
KW  - Dendritic Spines
KW  - Calcium: metabolism
KW  - Calcium (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40410447
C2  - pmc:PMC12102176
DO  - DOI:10.1038/s42003-025-08079-8
UR  - https://pub.dzne.de/record/278921
ER  -