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000279471 0247_ $$2doi$$a10.5061/DRYAD.TQJQ2BW90
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000279471 037__ $$aDZNE-2025-00798
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000279471 1001_ $$0P:(DE-2719)2811225$$aFuhrmann, Falko$$b0$$udzne
000279471 245__ $$aDataset: Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution
000279471 260__ $$bDryad$$c2025
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000279471 520__ $$aThe medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ±0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes’ motility (58.9 ±2% turnover rate) at previously unreachable depths (1100 µm). We establish three-photon imaging of the mPFC enabling neuronal and glial recordings with subcellular resolution that will pave the way for novel discoveries in this brain region.
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000279471 650_7 $$2Other$$aFOS: Natural sciences
000279471 650_7 $$2Other$$aThree-photon
000279471 650_7 $$2Other$$a3P
000279471 650_7 $$2Other$$atwo-photon
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000279471 650_7 $$2Other$$aIn vivo imaging
000279471 650_7 $$2Other$$amedial prefrontal cortex
000279471 650_7 $$2Other$$amPFC
000279471 650_7 $$2Other$$aSpinal cord
000279471 650_7 $$2Other$$aHippocampus
000279471 650_7 $$2Other$$aDentate gyrus
000279471 650_7 $$2Other$$aDG
000279471 650_7 $$2Other$$aCA1
000279471 650_7 $$2Other$$acranial window
000279471 650_7 $$2Other$$aopen skull
000279471 650_7 $$2Other$$ain vivo developmental imaging
000279471 650_7 $$2Other$$aintact cuticle
000279471 650_7 $$2Other$$aDrosophila
000279471 650_7 $$2Other$$amouse
000279471 650_7 $$2Other$$aMushroom body
000279471 650_7 $$2Other$$adendritic spines
000279471 650_7 $$2Other$$aGCaMP
000279471 650_7 $$2Other$$aCa2+-imaging
000279471 650_7 $$2Other$$aawake
000279471 650_7 $$2Other$$ahead-fixed
000279471 650_7 $$2Other$$aAstrocytes
000279471 650_7 $$2Other$$apyramidal neurons
000279471 650_7 $$2Other$$amicroglia
000279471 650_7 $$2Other$$amicroglial processes motility
000279471 650_7 $$2Other$$aTime-lapse
000279471 650_7 $$2Other$$a1300 nm
000279471 650_7 $$2Other$$a1650 nm
000279471 693__ $$0EXP:(DE-2719)LMF-20190308$$5EXP:(DE-2719)LMF-20190308$$eLight Microscope Facility (CRFS-LMF) / Bonn$$x0
000279471 7001_ $$0P:(DE-2719)2811414$$aNebeling, Felix$$b1$$udzne
000279471 7001_ $$0P:(DE-2719)2812689$$aMusacchio, Fabrizio$$b2$$udzne
000279471 7001_ $$0P:(DE-2719)2811044$$aMittag, Manuel$$b3$$udzne
000279471 7001_ $$0P:(DE-2719)2810397$$aPoll, Stefanie$$b4$$udzne
000279471 7001_ $$0P:(DE-2719)2811495$$aMüller, Monika$$b5
000279471 7001_ $$0P:(DE-2719)2811489$$aAmbrad Giovannetti, Eleonora$$b6$$udzne
000279471 7001_ $$0P:(DE-HGF)0$$aMaibach, Michael$$b7
000279471 7001_ $$0P:(DE-2719)2811123$$aSchaffran, Barbara$$b8$$udzne
000279471 7001_ $$0P:(DE-2719)9000509$$aBurnside, Emily$$b9$$udzne
000279471 7001_ $$0P:(DE-2719)9001354$$aChan, Chi Wai$$b10
000279471 7001_ $$0P:(DE-HGF)0$$aLagurin, Alex Simon$$b11
000279471 7001_ $$0P:(DE-2719)2811281$$aReichenbach, Nicole$$b12$$udzne
000279471 7001_ $$0P:(DE-2719)2812833$$aKaushalya, Sanjeev$$b13
000279471 7001_ $$0P:(DE-2719)2191568$$aFried, Hans$$b14$$udzne
000279471 7001_ $$0P:(DE-HGF)0$$aLinden, Stefan$$b15
000279471 7001_ $$0P:(DE-2719)2810273$$aPetzold, Gabor$$b16$$udzne
000279471 7001_ $$0P:(DE-2719)2810271$$aTavosanis, Gaia$$b17$$udzne
000279471 7001_ $$0P:(DE-2719)2810270$$aBradke, Frank$$b18$$udzne
000279471 7001_ $$0P:(DE-2719)2679991$$aFuhrmann, Martin$$b19
000279471 773__ $$a10.5061/dryad.tqjq2bw90
000279471 7870_ $$0DZNE-2025-00647$$aFuhrmann, Falko et.al.$$dLondon : Springer Nature, 2025$$iRelatedTo$$r$$tThree-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.
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