Dataset

DZNE-2025-00798

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution

Fuhrmann, F.DZNE* ; Nebeling, F.DZNE* ; Musacchio, F.DZNE* ; Mittag, M.DZNE* ; Poll, S.Extern* ; Müller, M.DZNE* ; Ambrad Giovannetti, E.DZNE* ; Maibach, M.DZNE* ; Schaffran, B.DZNE* ; Burnside, E.DZNE* ; Chan, C. W. ; Lagurin, A. S.DZNE* ; Reichenbach, N.DZNE* ; Kaushalya, S. ; Fried, H.DZNE* ; Linden, S. ; Petzold, G.DZNE* ; Tavosanis, G.Extern* ; Bradke, F.DZNE* ; Fuhrmann, M.DZNE*

2025
Dryad

This record in other databases:

Please use a persistent id in citations: doi:10.5061/DRYAD.TQJQ2BW90  doi:10.5061/dryad.tqjq2bw90

Abstract: The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ±0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes’ motility (58.9 ±2% turnover rate) at previously unreachable depths (1100 µm). We establish three-photon imaging of the mPFC enabling neuronal and glial recordings with subcellular resolution that will pave the way for novel discoveries in this brain region.

Keyword(s): FOS: Natural sciences ; Three-photon ; 3P ; two-photon ; 2P ; In vivo imaging ; medial prefrontal cortex ; mPFC ; Spinal cord ; Hippocampus ; Dentate gyrus ; DG ; CA1 ; cranial window ; open skull ; in vivo developmental imaging ; intact cuticle ; Drosophila ; mouse ; Mushroom body ; dendritic spines ; GCaMP ; Ca2+-imaging ; awake ; head-fixed ; Astrocytes ; pyramidal neurons ; microglia ; microglial processes motility ; Time-lapse ; 1300 nm ; 1650 nm

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Contributing Institute(s):

1. Neuroimmunology and Imaging (AG Fuhrmann)
2. Axon Growth and Regeneration (AG Bradke)
3. Nuclear Function in CNS Pathophysiology (AG Salomoni)
4. Vascular Neurology (AG Petzold)
5. Light Microscopy Facility (CRFS-LMF) (LMF)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)
2. 351 - Brain Function (POF4-351) (POF4-351)
3. 353 - Clinical and Health Care Research (POF4-353) (POF4-353)
4. 899 - ohne Topic (POF4-899) (POF4-899)

Experiment(s):

1. Light Microscope Facility (CRFS-LMF) / Bonn

Appears in the scientific report 2025

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Fuhrmann, F. (First author)DZNE* ; Nebeling, F. C. (First author)DZNE* ; Musacchio, F. (First author)DZNE* ; Mittag, M.DZNE* ; Poll, S.DZNE* ; Müller, M.DZNE* ; Ambrad Giovannetti, E.DZNE* ; Maibach, M.DZNE* ; Schaffran, B.DZNE* ; Burnside, E.DZNE* ; Chan, C. W.DZNE* ; Lagurin, A. S.DZNE* ; Reichenbach, N.DZNE* ; Kaushalya, S. K.DZNE* ; Fried, H.DZNE* ; Linden, S. ; Petzold, G. C.DZNE* ; Tavosanis, G.DZNE* ; Bradke, F.DZNE* ; Fuhrmann, M. (Last author)DZNE*
Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.
Communications biology 8(1), 795 (2025) [10.1038/s42003-025-08079-8]2025   Files  Fulltext by Pubmed Central BibTeX | EndNote: XML, Text | RIS

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