TY  - CHART
AU  - Fuhrmann, Falko
AU  - Nebeling, Felix
AU  - Musacchio, Fabrizio
AU  - Mittag, Manuel
AU  - Poll, Stefanie
AU  - Müller, Monika
AU  - Ambrad Giovannetti, Eleonora
AU  - Maibach, Michael
AU  - Schaffran, Barbara
AU  - Burnside, Emily
AU  - Chan, Chi Wai
AU  - Lagurin, Alex Simon
AU  - Reichenbach, Nicole
AU  - Kaushalya, Sanjeev
AU  - Fried, Hans
AU  - Linden, Stefan
AU  - Petzold, Gabor
AU  - Tavosanis, Gaia
AU  - Bradke, Frank
AU  - Fuhrmann, Martin
TI  - Dataset: Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution
PB  - Dryad
M1  - DZNE-2025-00798
PY  - 2025
AB  - The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ±0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes’ motility (58.9 ±2
KW  - FOS: Natural sciences (Other)
KW  - Three-photon (Other)
KW  - 3P (Other)
KW  - two-photon (Other)
KW  - 2P (Other)
KW  - In vivo imaging (Other)
KW  - medial prefrontal cortex (Other)
KW  - mPFC (Other)
KW  - Spinal cord (Other)
KW  - Hippocampus (Other)
KW  - Dentate gyrus (Other)
KW  - DG (Other)
KW  - CA1 (Other)
KW  - cranial window (Other)
KW  - open skull (Other)
KW  - in vivo developmental imaging (Other)
KW  - intact cuticle (Other)
KW  - Drosophila (Other)
KW  - mouse (Other)
KW  - Mushroom body (Other)
KW  - dendritic spines (Other)
KW  - GCaMP (Other)
KW  - Ca2+-imaging (Other)
KW  - awake (Other)
KW  - head-fixed (Other)
KW  - Astrocytes (Other)
KW  - pyramidal neurons (Other)
KW  - microglia (Other)
KW  - microglial processes motility (Other)
KW  - Time-lapse (Other)
KW  - 1300 nm (Other)
KW  - 1650 nm (Other)
LB  - PUB:(DE-HGF)32
DO  - DOI:10.5061/dryad.tqjq2bw90
UR  - https://pub.dzne.de/record/279471
ER  -