% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@MISC{Fuhrmann:279471,
      author       = {Fuhrmann, Falko and Nebeling, Felix and Musacchio, Fabrizio
                      and Mittag, Manuel and Poll, Stefanie and Müller, Monika
                      and Ambrad Giovannetti, Eleonora and Maibach, Michael and
                      Schaffran, Barbara and Burnside, Emily and Chan, Chi Wai and
                      Lagurin, Alex Simon and Reichenbach, Nicole and Kaushalya,
                      Sanjeev and Fried, Hans and Linden, Stefan and Petzold,
                      Gabor and Tavosanis, Gaia and Bradke, Frank and Fuhrmann,
                      Martin},
      title        = {{D}ataset: {T}hree-photon in vivo imaging of neurons and
                      glia in the medial prefrontal cortex with sub-cellular
                      resolution},
      publisher    = {Dryad},
      reportid     = {DZNE-2025-00798},
      year         = {2025},
      abstract     = {The medial prefrontal cortex (mPFC) is important for higher
                      cognitive functions, including working memory, decision
                      making, and emotional control. In vivo recordings of
                      neuronal activity in the mPFC have been achieved via
                      invasive electrical and optical approaches. Here we apply
                      low invasive three-photon in vivo imaging in the mPFC of the
                      mouse at unprecedented depth. Specifically, we measure
                      neuronal and astrocytic Ca2+-transient parameters in awake
                      head-fixed mice up to a depth of 1700 µm. Furthermore, we
                      longitudinally record dendritic spine density (0.41 ±0.07
                      µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength
                      to excite red fluorescent microglia, we quantify their
                      processes’ motility (58.9 $±2\%$ turnover rate) at
                      previously unreachable depths (1100 µm). We establish
                      three-photon imaging of the mPFC enabling neuronal and glial
                      recordings with subcellular resolution that will pave the
                      way for novel discoveries in this brain region.},
      keywords     = {FOS: Natural sciences (Other) / Three-photon (Other) / 3P
                      (Other) / two-photon (Other) / 2P (Other) / In vivo imaging
                      (Other) / medial prefrontal cortex (Other) / mPFC (Other) /
                      Spinal cord (Other) / Hippocampus (Other) / Dentate gyrus
                      (Other) / DG (Other) / CA1 (Other) / cranial window (Other)
                      / open skull (Other) / in vivo developmental imaging (Other)
                      / intact cuticle (Other) / Drosophila (Other) / mouse
                      (Other) / Mushroom body (Other) / dendritic spines (Other) /
                      GCaMP (Other) / Ca2+-imaging (Other) / awake (Other) /
                      head-fixed (Other) / Astrocytes (Other) / pyramidal neurons
                      (Other) / microglia (Other) / microglial processes motility
                      (Other) / Time-lapse (Other) / 1300 nm (Other) / 1650 nm
                      (Other)},
      cin          = {AG Fuhrmann / AG Bradke / AG Salomoni / AG Petzold / LMF},
      cid          = {I:(DE-2719)1011004 / I:(DE-2719)1013002 /
                      I:(DE-2719)1013032 / I:(DE-2719)1013020 /
                      I:(DE-2719)1040180},
      pnm          = {352 - Disease Mechanisms (POF4-352) / 351 - Brain Function
                      (POF4-351) / 353 - Clinical and Health Care Research
                      (POF4-353) / 899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-352 / G:(DE-HGF)POF4-351 /
                      G:(DE-HGF)POF4-353 / G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-2719)LMF-20190308},
      typ          = {PUB:(DE-HGF)32},
      doi          = {10.5061/dryad.tqjq2bw90},
      url          = {https://pub.dzne.de/record/279471},
}