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| Home > Publications Database > Dataset: Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution > print |
| 001 | 279471 | ||
| 005 | 20250710100950.0 | ||
| 024 | 7 | _ | |a 10.5061/DRYAD.TQJQ2BW90 |2 doi |
| 024 | 7 | _ | |a 10.5061/dryad.tqjq2bw90 |2 doi |
| 037 | _ | _ | |a DZNE-2025-00798 |
| 041 | _ | _ | |a English |
| 100 | 1 | _ | |a Fuhrmann, Falko |0 P:(DE-2719)2811225 |b 0 |u dzne |
| 245 | _ | _ | |a Dataset: Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution |
| 260 | _ | _ | |c 2025 |b Dryad |
| 336 | 7 | _ | |a MISC |2 BibTeX |
| 336 | 7 | _ | |a Dataset |b dataset |m dataset |0 PUB:(DE-HGF)32 |s 1752050123_18333 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Chart or Table |0 26 |2 EndNote |
| 336 | 7 | _ | |a Dataset |2 DataCite |
| 336 | 7 | _ | |a DATA_SET |2 ORCID |
| 336 | 7 | _ | |a ResearchData |2 DINI |
| 520 | _ | _ | |a The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca2+-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ±0.07 µm-1) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes’ motility (58.9 ±2% turnover rate) at previously unreachable depths (1100 µm). We establish three-photon imaging of the mPFC enabling neuronal and glial recordings with subcellular resolution that will pave the way for novel discoveries in this brain region. |
| 536 | _ | _ | |a 352 - Disease Mechanisms (POF4-352) |0 G:(DE-HGF)POF4-352 |c POF4-352 |f POF IV |x 0 |
| 536 | _ | _ | |a 351 - Brain Function (POF4-351) |0 G:(DE-HGF)POF4-351 |c POF4-351 |f POF IV |x 1 |
| 536 | _ | _ | |a 353 - Clinical and Health Care Research (POF4-353) |0 G:(DE-HGF)POF4-353 |c POF4-353 |f POF IV |x 2 |
| 536 | _ | _ | |a 899 - ohne Topic (POF4-899) |0 G:(DE-HGF)POF4-899 |c POF4-899 |f POF IV |x 3 |
| 588 | _ | _ | |a Dataset connected to DataCite |
| 650 | _ | 7 | |a FOS: Natural sciences |2 Other |
| 650 | _ | 7 | |a Three-photon |2 Other |
| 650 | _ | 7 | |a 3P |2 Other |
| 650 | _ | 7 | |a two-photon |2 Other |
| 650 | _ | 7 | |a 2P |2 Other |
| 650 | _ | 7 | |a In vivo imaging |2 Other |
| 650 | _ | 7 | |a medial prefrontal cortex |2 Other |
| 650 | _ | 7 | |a mPFC |2 Other |
| 650 | _ | 7 | |a Spinal cord |2 Other |
| 650 | _ | 7 | |a Hippocampus |2 Other |
| 650 | _ | 7 | |a Dentate gyrus |2 Other |
| 650 | _ | 7 | |a DG |2 Other |
| 650 | _ | 7 | |a CA1 |2 Other |
| 650 | _ | 7 | |a cranial window |2 Other |
| 650 | _ | 7 | |a open skull |2 Other |
| 650 | _ | 7 | |a in vivo developmental imaging |2 Other |
| 650 | _ | 7 | |a intact cuticle |2 Other |
| 650 | _ | 7 | |a Drosophila |2 Other |
| 650 | _ | 7 | |a mouse |2 Other |
| 650 | _ | 7 | |a Mushroom body |2 Other |
| 650 | _ | 7 | |a dendritic spines |2 Other |
| 650 | _ | 7 | |a GCaMP |2 Other |
| 650 | _ | 7 | |a Ca2+-imaging |2 Other |
| 650 | _ | 7 | |a awake |2 Other |
| 650 | _ | 7 | |a head-fixed |2 Other |
| 650 | _ | 7 | |a Astrocytes |2 Other |
| 650 | _ | 7 | |a pyramidal neurons |2 Other |
| 650 | _ | 7 | |a microglia |2 Other |
| 650 | _ | 7 | |a microglial processes motility |2 Other |
| 650 | _ | 7 | |a Time-lapse |2 Other |
| 650 | _ | 7 | |a 1300 nm |2 Other |
| 650 | _ | 7 | |a 1650 nm |2 Other |
| 693 | _ | _ | |0 EXP:(DE-2719)LMF-20190308 |5 EXP:(DE-2719)LMF-20190308 |e Light Microscope Facility (CRFS-LMF) / Bonn |x 0 |
| 700 | 1 | _ | |a Nebeling, Felix |0 P:(DE-2719)2811414 |b 1 |u dzne |
| 700 | 1 | _ | |a Musacchio, Fabrizio |0 P:(DE-2719)2812689 |b 2 |u dzne |
| 700 | 1 | _ | |a Mittag, Manuel |0 P:(DE-2719)2811044 |b 3 |u dzne |
| 700 | 1 | _ | |a Poll, Stefanie |0 P:(DE-2719)2810397 |b 4 |u dzne |
| 700 | 1 | _ | |a Müller, Monika |0 P:(DE-2719)2811495 |b 5 |
| 700 | 1 | _ | |a Ambrad Giovannetti, Eleonora |0 P:(DE-2719)2811489 |b 6 |u dzne |
| 700 | 1 | _ | |a Maibach, Michael |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Schaffran, Barbara |0 P:(DE-2719)2811123 |b 8 |u dzne |
| 700 | 1 | _ | |a Burnside, Emily |0 P:(DE-2719)9000509 |b 9 |u dzne |
| 700 | 1 | _ | |a Chan, Chi Wai |0 P:(DE-2719)9001354 |b 10 |
| 700 | 1 | _ | |a Lagurin, Alex Simon |0 P:(DE-HGF)0 |b 11 |
| 700 | 1 | _ | |a Reichenbach, Nicole |0 P:(DE-2719)2811281 |b 12 |u dzne |
| 700 | 1 | _ | |a Kaushalya, Sanjeev |0 P:(DE-2719)2812833 |b 13 |
| 700 | 1 | _ | |a Fried, Hans |0 P:(DE-2719)2191568 |b 14 |u dzne |
| 700 | 1 | _ | |a Linden, Stefan |0 P:(DE-HGF)0 |b 15 |
| 700 | 1 | _ | |a Petzold, Gabor |0 P:(DE-2719)2810273 |b 16 |u dzne |
| 700 | 1 | _ | |a Tavosanis, Gaia |0 P:(DE-2719)2810271 |b 17 |u dzne |
| 700 | 1 | _ | |a Bradke, Frank |0 P:(DE-2719)2810270 |b 18 |u dzne |
| 700 | 1 | _ | |a Fuhrmann, Martin |0 P:(DE-2719)2679991 |b 19 |
| 773 | _ | _ | |a 10.5061/dryad.tqjq2bw90 |
| 787 | 0 | _ | |a Fuhrmann, Falko et.al. |d London : Springer Nature, 2025 |i RelatedTo |0 DZNE-2025-00647 |r |t Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution. |
| 909 | C | O | |o oai:pub.dzne.de:279471 |p VDB |
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| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Neurodegenerative Diseases |1 G:(DE-HGF)POF4-350 |0 G:(DE-HGF)POF4-352 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-300 |4 G:(DE-HGF)POF |v Disease Mechanisms |x 0 |
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| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Neurodegenerative Diseases |1 G:(DE-HGF)POF4-350 |0 G:(DE-HGF)POF4-353 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-300 |4 G:(DE-HGF)POF |v Clinical and Health Care Research |x 2 |
| 913 | 1 | _ | |a DE-HGF |b Programmungebundene Forschung |l ohne Programm |1 G:(DE-HGF)POF4-890 |0 G:(DE-HGF)POF4-899 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-800 |4 G:(DE-HGF)POF |v ohne Topic |x 3 |
| 914 | 1 | _ | |y 2025 |
| 920 | 1 | _ | |0 I:(DE-2719)1011004 |k AG Fuhrmann |l Neuroimmunology and Imaging |x 0 |
| 920 | 1 | _ | |0 I:(DE-2719)1013002 |k AG Bradke |l Axon Growth and Regeneration |x 1 |
| 920 | 1 | _ | |0 I:(DE-2719)1013032 |k AG Salomoni |l Nuclear Function in CNS Pathophysiology |x 2 |
| 920 | 1 | _ | |0 I:(DE-2719)1013020 |k AG Petzold |l Vascular Neurology |x 3 |
| 920 | 1 | _ | |0 I:(DE-2719)1040180 |k LMF |l Light Microscopy Facility (CRFS-LMF) |x 4 |
| 980 | _ | _ | |a dataset |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-2719)1011004 |
| 980 | _ | _ | |a I:(DE-2719)1013002 |
| 980 | _ | _ | |a I:(DE-2719)1013032 |
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| 980 | _ | _ | |a I:(DE-2719)1040180 |
| 980 | _ | _ | |a UNRESTRICTED |
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