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@MISC{Schultze:279476,
author = {Schultze, Joachim and de Domenico, Elena and Saglam, Adem
and Drews, Anna-Dorothee and Ulas, Thomas and Becker,
Matthias Kai Holger and Händler, Kristian},
title = {{D}ataset: {S}evere {COVID}-19 {I}s {M}arked by a
{D}ysregulated {M}yeloid {C}ell {C}ompartment},
publisher = {Human Cell Atlas Data Explorer},
reportid = {DZNE-2025-00803},
year = {2025},
abstract = {Coronavirus disease 2019 (COVID-19) is a mild to moderate
respiratory tract infection, however, a subset of patients
progress to severe disease and respiratory failure. The
mechanism of protective immunity in mild forms and the
pathogenesis of severe COVID-19 associated with increased
neutrophil counts and dysregulated immune responses remain
unclear. In a dual-center, two-cohort study, we combined
single-cell RNA-sequencing and single-cell proteomics of
whole-blood and peripheral-blood mononuclear cells to
determine changes in immune cell composition and activation
in mild versus severe COVID-19 (242 samples from 109
individuals) over time. HLA-DRhiCD11chi inflammatory
monocytes with an interferon-stimulated gene signature were
elevated in mild COVID-19. Severe COVID-19 was marked by
occurrence of neutrophil precursors, as evidence of
emergency myelopoiesis, dysfunctional mature neutrophils,
and HLA-DRlo monocytes. Our study provides detailed insights
into the systemic immune response to SARS-CoV-2 infection
and reveals profound alterations in the myeloid cell
compartment associated with severe COVID-19.},
cin = {AG Schultze},
cid = {I:(DE-2719)1013038},
pnm = {354 - Disease Prevention and Healthy Aging (POF4-354)},
pid = {G:(DE-HGF)POF4-354},
typ = {PUB:(DE-HGF)32},
url = {https://pub.dzne.de/record/279476},
}