000279479 001__ 279479
000279479 005__ 20250710100950.0
000279479 037__ $$aDZNE-2025-00806
000279479 1001_ $$0P:(DE-2719)2810938$$aMüller, Stephan A$$b0$$udzne
000279479 245__ $$aDataset: Quantitative proteomics of CSF from the Alzheimer's disease mouse model 5xFAD
000279479 260__ $$bPRoteomics IDEntifications Database$$c2025
000279479 3367_ $$2BibTeX$$aMISC
000279479 3367_ $$0PUB:(DE-HGF)32$$2PUB:(DE-HGF)$$aDataset$$bdataset$$mdataset$$s1752049604_18284
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000279479 520__ $$aThe 5xFAD mouse model was used to study the influence of amyloid pathology on the cerebrospinal fluid (CSF) proteome. Therefore, CSF of 7-months old 5xFAD and WT control mice was sampled with an improved collection protocol. Using a minute amount of 5 µL CSF, we applied data dependent and data independent acquisition of the same samples on a Q-Exactive HF mass spectrometer. Comparison of DDA and DIA demonstrate a higher data completeness for DIA. The results show a strong effect on microglia activation exemplified by strongly increased abundances of Trem2 and Cst7. Furthermore, neurodegenerative markers such as neurofilaments and tau (Mapt) were strongly increased for 5xFAD mice.
000279479 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000279479 7001_ $$0P:(DE-2719)2181459$$aLichtenthaler, Stefan$$b1$$udzne
000279479 7870_ $$0DZNE-2025-00672$$aLourbopoulos, Athanasios et.al.$$dBethesda, Md. : The American Society for Biochemistry and Molecular Biology, 2025$$iRelatedTo$$r$$tAn Improved Method for Sampling and Quantitative Protein Analytics of Cerebrospinal Fluid of Individual Mice.
000279479 8564_ $$uhttps://wwwdev.ebi.ac.uk/pride/archive/projects/PXD053568
000279479 909CO $$ooai:pub.dzne.de:279479$$pVDB
000279479 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810938$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000279479 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2181459$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000279479 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000279479 9141_ $$y2025
000279479 9201_ $$0I:(DE-2719)1110006$$kAG Lichtenthaler$$lNeuroproteomics$$x0
000279479 980__ $$adataset
000279479 980__ $$aVDB
000279479 980__ $$aI:(DE-2719)1110006
000279479 980__ $$aUNRESTRICTED