%0 Journal Article
%A Armăşescu, Florian
%A Amuzescu, Bogdan
%A Gheorghe, Roxana-Olimpia
%A Ghenghea, Mihail
%A Ristoiu, Violeta
%A Ciurea, Jean
%A Gruia, Ion
%T Fiber-optic-guided near-infrared laser exposure induces depolarization of cultured primary sensory neurons and modifies biophysical properties of human Nav1.5 channels.
%J Journal of photochemistry and photobiology / B
%V 269
%@ 1011-1344
%C New York, NY [u.a.]
%I Elsevier
%M DZNE-2025-00851
%P 113191
%D 2025
%X Photobiomodulation, a therapeutic method promoting wound healing, reduction in inflammation, pain and apoptosis, was widely tested in neurological/psychiatric disorders. In Parkinson's disease positive results have been obtained recently by transcranial or deep-fiber-optic-based near-infrared (NIR) light application. We assessed the effects of NIR stimulation with a 808.5 nm diode laser applied via a multimode fiber with a sharp tip placed over the cell on enzyme-dissociated cultured adult rat primary sensory neurons and human embryo kidney (HEK293) cells stably expressing human voltage-dependent Na+ channels (Nav1.5) approached via patch-clamp. For each type of cell, specific series of voltage- or current-clamp protocols were applied initially and after 3 min of laser exposure or control conditions. Laser exposure induced in neurons a resting potential depolarization (6.6 ± 1.8 mV vs. 2.4 ± 1.8 mV in control, mean ± SEM, p = 0.0594). In Nav1.5-expressing cells, peak INa amplitude slightly increased after laser application (111.2 ± 14.9 
%K Humans
%K HEK293 Cells
%K NAV1.5 Voltage-Gated Sodium Channel: metabolism
%K Infrared Rays
%K Rats
%K Animals
%K Sensory Receptor Cells: radiation effects
%K Sensory Receptor Cells: metabolism
%K Sensory Receptor Cells: cytology
%K Lasers
%K Cells, Cultured
%K Patch-Clamp Techniques
%K Membrane Potentials: radiation effects
%K NIR laser (Other)
%K Nav1.5 (Other)
%K Patch-clamp (Other)
%K Phosphorylation (Other)
%K Photobiomodulation (Other)
%K Primary sensory neuron (Other)
%K NAV1.5 Voltage-Gated Sodium Channel (NLM Chemicals)
%K SCN5A protein, human (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40460513
%R 10.1016/j.jphotobiol.2025.113191
%U https://pub.dzne.de/record/279884