guest ::
| Home > Publications Database > Fiber-optic-guided near-infrared laser exposure induces depolarization of cultured primary sensory neurons and modifies biophysical properties of human Nav1.5 channels. |
| Home > Publications Database > Fiber-optic-guided near-infrared laser exposure induces depolarization of cultured primary sensory neurons and modifies biophysical properties of human Nav1.5 channels. |
| Journal Article | DZNE-2025-00851 |
; ; ; ; ; ;
2025
Elsevier
New York, NY [u.a.]
This record in other databases: 
Please use a persistent id in citations: doi:10.1016/j.jphotobiol.2025.113191
Abstract: Photobiomodulation, a therapeutic method promoting wound healing, reduction in inflammation, pain and apoptosis, was widely tested in neurological/psychiatric disorders. In Parkinson's disease positive results have been obtained recently by transcranial or deep-fiber-optic-based near-infrared (NIR) light application. We assessed the effects of NIR stimulation with a 808.5 nm diode laser applied via a multimode fiber with a sharp tip placed over the cell on enzyme-dissociated cultured adult rat primary sensory neurons and human embryo kidney (HEK293) cells stably expressing human voltage-dependent Na+ channels (Nav1.5) approached via patch-clamp. For each type of cell, specific series of voltage- or current-clamp protocols were applied initially and after 3 min of laser exposure or control conditions. Laser exposure induced in neurons a resting potential depolarization (6.6 ± 1.8 mV vs. 2.4 ± 1.8 mV in control, mean ± SEM, p = 0.0594). In Nav1.5-expressing cells, peak INa amplitude slightly increased after laser application (111.2 ± 14.9 % vs. 70.6 ± 10.4 % in control experiments), and in outside-out patches the differences were larger (96.64 ± 5.25 %-laser vs. 37.95 ± 9.14 %-control). Via chemiluminometry we evidenced a delayed increase in ATP production in laser-exposed HEK293 cells. An explanation of these effects is that NIR exposure facilitates ATP production, maintaining an adequate state of Na+ channels phosphorylation, but we cannot exclude direct polarization effects on macromolecules including ion channels produced by the intense oriented electric field of the laser beam.
Keyword(s): Humans (MeSH) ; HEK293 Cells (MeSH) ; NAV1.5 Voltage-Gated Sodium Channel: metabolism (MeSH) ; Infrared Rays (MeSH) ; Rats (MeSH) ; Animals (MeSH) ; Sensory Receptor Cells: radiation effects (MeSH) ; Sensory Receptor Cells: metabolism (MeSH) ; Sensory Receptor Cells: cytology (MeSH) ; Lasers (MeSH) ; Cells, Cultured (MeSH) ; Patch-Clamp Techniques (MeSH) ; Membrane Potentials: radiation effects (MeSH) ; NIR laser ; Nav1.5 ; Patch-clamp ; Phosphorylation ; Photobiomodulation ; Primary sensory neuron ; NAV1.5 Voltage-Gated Sodium Channel ; SCN5A protein, human
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz
; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record
|
The record appears in these collections: |
DOCX
PDF