TY  - JOUR
AU  - Armăşescu, Florian
AU  - Amuzescu, Bogdan
AU  - Gheorghe, Roxana-Olimpia
AU  - Ghenghea, Mihail
AU  - Ristoiu, Violeta
AU  - Ciurea, Jean
AU  - Gruia, Ion
TI  - Fiber-optic-guided near-infrared laser exposure induces depolarization of cultured primary sensory neurons and modifies biophysical properties of human Nav1.5 channels.
JO  - Journal of photochemistry and photobiology / B
VL  - 269
SN  - 1011-1344
CY  - New York, NY [u.a.]
PB  - Elsevier
M1  - DZNE-2025-00851
SP  - 113191
PY  - 2025
AB  - Photobiomodulation, a therapeutic method promoting wound healing, reduction in inflammation, pain and apoptosis, was widely tested in neurological/psychiatric disorders. In Parkinson's disease positive results have been obtained recently by transcranial or deep-fiber-optic-based near-infrared (NIR) light application. We assessed the effects of NIR stimulation with a 808.5 nm diode laser applied via a multimode fiber with a sharp tip placed over the cell on enzyme-dissociated cultured adult rat primary sensory neurons and human embryo kidney (HEK293) cells stably expressing human voltage-dependent Na+ channels (Nav1.5) approached via patch-clamp. For each type of cell, specific series of voltage- or current-clamp protocols were applied initially and after 3 min of laser exposure or control conditions. Laser exposure induced in neurons a resting potential depolarization (6.6 ± 1.8 mV vs. 2.4 ± 1.8 mV in control, mean ± SEM, p = 0.0594). In Nav1.5-expressing cells, peak INa amplitude slightly increased after laser application (111.2 ± 14.9 
KW  - Humans
KW  - HEK293 Cells
KW  - NAV1.5 Voltage-Gated Sodium Channel: metabolism
KW  - Infrared Rays
KW  - Rats
KW  - Animals
KW  - Sensory Receptor Cells: radiation effects
KW  - Sensory Receptor Cells: metabolism
KW  - Sensory Receptor Cells: cytology
KW  - Lasers
KW  - Cells, Cultured
KW  - Patch-Clamp Techniques
KW  - Membrane Potentials: radiation effects
KW  - NIR laser (Other)
KW  - Nav1.5 (Other)
KW  - Patch-clamp (Other)
KW  - Phosphorylation (Other)
KW  - Photobiomodulation (Other)
KW  - Primary sensory neuron (Other)
KW  - NAV1.5 Voltage-Gated Sodium Channel (NLM Chemicals)
KW  - SCN5A protein, human (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40460513
DO  - DOI:10.1016/j.jphotobiol.2025.113191
UR  - https://pub.dzne.de/record/279884
ER  -