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@ARTICLE{Armescu:279884,
      author       = {Armăşescu, Florian and Amuzescu, Bogdan and Gheorghe,
                      Roxana-Olimpia and Ghenghea, Mihail and Ristoiu, Violeta and
                      Ciurea, Jean and Gruia, Ion},
      title        = {{F}iber-optic-guided near-infrared laser exposure induces
                      depolarization of cultured primary sensory neurons and
                      modifies biophysical properties of human {N}av1.5 channels.},
      journal      = {Journal of photochemistry and photobiology / B},
      volume       = {269},
      issn         = {1011-1344},
      address      = {New York, NY [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DZNE-2025-00851},
      pages        = {113191},
      year         = {2025},
      abstract     = {Photobiomodulation, a therapeutic method promoting wound
                      healing, reduction in inflammation, pain and apoptosis, was
                      widely tested in neurological/psychiatric disorders. In
                      Parkinson's disease positive results have been obtained
                      recently by transcranial or deep-fiber-optic-based
                      near-infrared (NIR) light application. We assessed the
                      effects of NIR stimulation with a 808.5 nm diode laser
                      applied via a multimode fiber with a sharp tip placed over
                      the cell on enzyme-dissociated cultured adult rat primary
                      sensory neurons and human embryo kidney (HEK293) cells
                      stably expressing human voltage-dependent Na+ channels
                      (Nav1.5) approached via patch-clamp. For each type of cell,
                      specific series of voltage- or current-clamp protocols were
                      applied initially and after 3 min of laser exposure or
                      control conditions. Laser exposure induced in neurons a
                      resting potential depolarization (6.6 ± 1.8 mV vs. 2.4 ±
                      1.8 mV in control, mean ± SEM, p = 0.0594). In
                      Nav1.5-expressing cells, peak INa amplitude slightly
                      increased after laser application (111.2 ± 14.9 $\%$ vs.
                      70.6 ± 10.4 $\%$ in control experiments), and in
                      outside-out patches the differences were larger (96.64 ±
                      5.25 $\%-laser$ vs. 37.95 ± 9.14 $\%-control).$ Via
                      chemiluminometry we evidenced a delayed increase in ATP
                      production in laser-exposed HEK293 cells. An explanation of
                      these effects is that NIR exposure facilitates ATP
                      production, maintaining an adequate state of Na+ channels
                      phosphorylation, but we cannot exclude direct polarization
                      effects on macromolecules including ion channels produced by
                      the intense oriented electric field of the laser beam.},
      keywords     = {Humans / HEK293 Cells / NAV1.5 Voltage-Gated Sodium
                      Channel: metabolism / Infrared Rays / Rats / Animals /
                      Sensory Receptor Cells: radiation effects / Sensory Receptor
                      Cells: metabolism / Sensory Receptor Cells: cytology /
                      Lasers / Cells, Cultured / Patch-Clamp Techniques / Membrane
                      Potentials: radiation effects / NIR laser (Other) / Nav1.5
                      (Other) / Patch-clamp (Other) / Phosphorylation (Other) /
                      Photobiomodulation (Other) / Primary sensory neuron (Other)
                      / NAV1.5 Voltage-Gated Sodium Channel (NLM Chemicals) /
                      SCN5A protein, human (NLM Chemicals)},
      cin          = {AG Dityatev},
      ddc          = {540},
      cid          = {I:(DE-2719)1310007},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40460513},
      doi          = {10.1016/j.jphotobiol.2025.113191},
      url          = {https://pub.dzne.de/record/279884},
}