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000281188 1001_ $$aFlaskamp, Lavinia$$b0
000281188 245__ $$aAssessing Extracellular Vesicle Turnover In Vivo Using Highly Sensitive Phosphatidylserine‐Binding Reagents
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000281188 520__ $$aExtracellular vesicles (EVs) are emerging as crucial players in cell communication and hold great promise as biomarkers and therapeutic tools. However, their diversity makes it challenging to detect, classify, and utilize them effectively, which limits their clinical applicability. A key challenge is the lack of reliable markers to identify EVs consistently. In this study, a novel high-affinity phosphatidylserine (PS)-binding reagent is introduced for EV analysis. PS is known as a marker of apoptotic cells and activated platelets, but its presence on EVs is debated due to variations in lipid composition. By comparing multiple PS-binding reagents, including MFG-E8 derivatives and Annexin V, it is demonstrated that ≈90% of EVs in human and mouse blood carry PS. Using the optimized reagent, the first in vivo insights into EV turnover are provided, showing that PS+ EVs in mouse blood are rapidly cleared (≈50% within 30 min) but persist on immune cells in the spleen. This discovery increases the potential of EVs as disease biomarkers and therapeutic targets by improving EV detection and isolation as well as opening the door for standardized quantification and diagnostic monitoring.
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000281188 650_7 $$2Other$$aEV‐turnover
000281188 650_7 $$2Other$$aMFG‐E8
000281188 650_7 $$2Other$$aextracellular Vesicles (EVs)
000281188 650_7 $$2Other$$alactadherin
000281188 650_7 $$2Other$$aphosphatidylserine (PS)
000281188 650_7 $$2NLM Chemicals$$aPhosphatidylserines
000281188 650_7 $$2NLM Chemicals$$aBiomarkers
000281188 650_7 $$2NLM Chemicals$$aAnnexin A5
000281188 650_2 $$2MeSH$$aExtracellular Vesicles: metabolism
000281188 650_2 $$2MeSH$$aPhosphatidylserines: metabolism
000281188 650_2 $$2MeSH$$aAnimals
000281188 650_2 $$2MeSH$$aMice
000281188 650_2 $$2MeSH$$aHumans
000281188 650_2 $$2MeSH$$aBiomarkers: metabolism
000281188 650_2 $$2MeSH$$aMice, Inbred C57BL
000281188 650_2 $$2MeSH$$aAnnexin A5: metabolism
000281188 7001_ $$aPrechtl, Monica$$b1
000281188 7001_ $$aScheck, Annkathrin$$b2
000281188 7001_ $$aHu, Wenbo$$b3
000281188 7001_ $$aRied, Christine$$b4
000281188 7001_ $$0P:(DE-2719)9000614$$aKislinger, Georg$$b5$$udzne
000281188 7001_ $$0P:(DE-2719)2811642$$aSimons, Mikael$$b6$$udzne
000281188 7001_ $$aKrug, Anne B.$$b7
000281188 7001_ $$aKranich, Jan$$b8
000281188 7001_ $$00000-0001-7060-5433$$aBrocker, Thomas$$b9
000281188 773__ $$0PERI:(DE-600)2808093-2$$a10.1002/advs.202507624$$gp. e07624$$n40$$pe07624$$tAdvanced science$$v12$$x2198-3844$$y2025
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