000282939 001__ 282939
000282939 005__ 20251218103447.0
000282939 0247_ $$2doi$$a10.17632/W97NRDVXBC.1
000282939 0247_ $$2doi$$a10.17632/w97nrdvxbc.1
000282939 037__ $$aDZNE-2025-01400
000282939 1001_ $$aKroon, Cristina$$b0
000282939 245__ $$aDataset: Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release
000282939 260__ $$bMendeley Data$$c2025
000282939 3367_ $$2BibTeX$$aMISC
000282939 3367_ $$0PUB:(DE-HGF)32$$2PUB:(DE-HGF)$$aDataset$$bdataset$$mdataset$$s1765985150_5348
000282939 3367_ $$026$$2EndNote$$aChart or Table
000282939 3367_ $$2DataCite$$aDataset
000282939 3367_ $$2ORCID$$aDATA_SET
000282939 3367_ $$2DINI$$aResearchData
000282939 520__ $$aThis dataset contains the raw data from the publication „Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release“ Kroon et al. 2025 published in iScience. Presented here are the original western and PhosTag blots supporting the biochemical characterization, as well as mass spectrometry and immunocytochemistry data. For further details please refer to the publication. Abstract: Phospholipid-phosphatase related protein 3 (PLPPR3) belongs to a family of transmembrane proteins highly expressed in the nervous system where it regulates critical axonal growth processes during guidance, filopodia formation and branching. However, little is known regarding its role in synapses and the signaling events regulating PLPPR3 function. Here, we identify 26 high-confidence phosphorylation sites in the intracellular domain of PLPPR3 using mass spectrometry. Biochemical characterization established one of these – S351 – as a bona fide phosphorylation site of protein kinase A (PKA). PLPPR3 is enriched at presynaptic terminals, and deletion of PLPPR3 results in increased depolarization-induced synaptic vesicle release in hippocampal neurons. This tonic inhibitory signal towards depolarization-induced presynaptic activity is corrected by expression of PLPPR3 intracellular domain, but not a S351A phospho-dead mutant, in Plppr3-/- hippocampal neurons. We propose that a PLPPR3 phosphorylation under the control of PKA activity is a novel signaling integrator of presynaptic activity in hippocampal neurons.
000282939 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x0
000282939 588__ $$aDataset connected to DataCite
000282939 650_7 $$2Other$$aMolecular Neuroscience
000282939 650_7 $$2Other$$aPhosphorylation
000282939 650_7 $$2Other$$aProtein-Protein Interaction
000282939 650_7 $$2Other$$aSignal Transduction
000282939 650_7 $$2Other$$aCellular Neuroscience
000282939 650_7 $$2Other$$aPresynaptic Mechanisms of Synaptic Transmission
000282939 650_7 $$2Other$$aProtein Phosphorylation
000282939 7001_ $$aNagy-Herczeg, Domonkos$$b1
000282939 7001_ $$0P:(DE-2719)9002012$$aAguilar Perez, Gerard$$b2$$udzne
000282939 7001_ $$aRanti, Dimitra$$b3
000282939 7001_ $$aSyropoulou, Vasiliki$$b4
000282939 773__ $$a10.17632/w97nrdvxbc.1
000282939 7870_ $$0DZNE-2025-01117$$aKroon, Cristina et.al.$$dSt. Louis : Elsevier, 2025$$iRelatedTo$$r$$tPhosphorylation of presynaptic PLPPR3 controls synaptic vesicle release
000282939 909CO $$ooai:pub.dzne.de:282939$$pVDB
000282939 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9002012$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b2$$kDZNE
000282939 9131_ $$0G:(DE-HGF)POF4-351$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vBrain Function$$x0
000282939 9141_ $$y2025
000282939 9201_ $$0I:(DE-2719)1813002$$kAG Milovanovic (Berlin)$$lMolecular Neuroscience$$x0
000282939 980__ $$adataset
000282939 980__ $$aVDB
000282939 980__ $$aI:(DE-2719)1813002
000282939 980__ $$aUNRESTRICTED