Dataset

DZNE-2025-01400

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release

Kroon, C. ; Nagy-Herczeg, D. ; Aguilar Perez, G.DZNE* ; Ranti, D. ; Syropoulou, V.

2025
Mendeley Data

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Please use a persistent id in citations: doi:10.17632/w97nrdvxbc.1  doi:10.17632/W97NRDVXBC.1

Abstract: This dataset contains the raw data from the publication „Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release“ Kroon et al. 2025 published in iScience. Presented here are the original western and PhosTag blots supporting the biochemical characterization, as well as mass spectrometry and immunocytochemistry data. For further details please refer to the publication. Abstract: Phospholipid-phosphatase related protein 3 (PLPPR3) belongs to a family of transmembrane proteins highly expressed in the nervous system where it regulates critical axonal growth processes during guidance, filopodia formation and branching. However, little is known regarding its role in synapses and the signaling events regulating PLPPR3 function. Here, we identify 26 high-confidence phosphorylation sites in the intracellular domain of PLPPR3 using mass spectrometry. Biochemical characterization established one of these – S351 – as a bona fide phosphorylation site of protein kinase A (PKA). PLPPR3 is enriched at presynaptic terminals, and deletion of PLPPR3 results in increased depolarization-induced synaptic vesicle release in hippocampal neurons. This tonic inhibitory signal towards depolarization-induced presynaptic activity is corrected by expression of PLPPR3 intracellular domain, but not a S351A phospho-dead mutant, in Plppr3-/- hippocampal neurons. We propose that a PLPPR3 phosphorylation under the control of PKA activity is a novel signaling integrator of presynaptic activity in hippocampal neurons.

Keyword(s): Molecular Neuroscience ; Phosphorylation ; Protein-Protein Interaction ; Signal Transduction ; Cellular Neuroscience ; Presynaptic Mechanisms of Synaptic Transmission ; Protein Phosphorylation

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Contributing Institute(s):

1. Molecular Neuroscience (AG Milovanovic (Berlin))

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2025

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Kroon, C. ; Bareesel, S. ; Perez, G. A.DZNE* ; Nagy-Herczeg, D. ; Ranti, D. ; Syropoulou, V. ; Coveney, S. ; Kirchner, M. ; Gimber, N. ; Bintig, W. ; Brosig, A. ; Braune, G. ; Textoris-Taube, K. ; Zolnik, T. A. ; Mertins, P. ; Schmoranzer, J. ; Milovanovic, D.DZNE* ; Leondaritis, G. ; Eickholt, B. J.
Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release
iScience 28(9), 113435 (2025) [10.1016/j.isci.2025.113435]2025   Files BibTeX | EndNote: XML, Text | RIS

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