Home > Publications Database > Dataset: Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release > print

001     282939
005     20251218103447.0
024 7 _ |a 10.17632/W97NRDVXBC.1
|2 doi
024 7 _ |a 10.17632/w97nrdvxbc.1
|2 doi
037 _ _ |a DZNE-2025-01400
100 1 _ |a Kroon, Cristina
|b 0
245 _ _ |a Dataset: Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release
260 _ _ |c 2025
|b Mendeley Data
336 7 _ |a MISC
|2 BibTeX
336 7 _ |a Dataset
|b dataset
|m dataset
|0 PUB:(DE-HGF)32
|s 1765985150_5348
|2 PUB:(DE-HGF)
336 7 _ |a Chart or Table
|0 26
|2 EndNote
336 7 _ |a Dataset
|2 DataCite
336 7 _ |a DATA_SET
|2 ORCID
336 7 _ |a ResearchData
|2 DINI
520 _ _ |a This dataset contains the raw data from the publication „Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release“ Kroon et al. 2025 published in iScience. Presented here are the original western and PhosTag blots supporting the biochemical characterization, as well as mass spectrometry and immunocytochemistry data. For further details please refer to the publication. Abstract: Phospholipid-phosphatase related protein 3 (PLPPR3) belongs to a family of transmembrane proteins highly expressed in the nervous system where it regulates critical axonal growth processes during guidance, filopodia formation and branching. However, little is known regarding its role in synapses and the signaling events regulating PLPPR3 function. Here, we identify 26 high-confidence phosphorylation sites in the intracellular domain of PLPPR3 using mass spectrometry. Biochemical characterization established one of these – S351 – as a bona fide phosphorylation site of protein kinase A (PKA). PLPPR3 is enriched at presynaptic terminals, and deletion of PLPPR3 results in increased depolarization-induced synaptic vesicle release in hippocampal neurons. This tonic inhibitory signal towards depolarization-induced presynaptic activity is corrected by expression of PLPPR3 intracellular domain, but not a S351A phospho-dead mutant, in Plppr3-/- hippocampal neurons. We propose that a PLPPR3 phosphorylation under the control of PKA activity is a novel signaling integrator of presynaptic activity in hippocampal neurons.
536 _ _ |a 351 - Brain Function (POF4-351)
|0 G:(DE-HGF)POF4-351
|c POF4-351
|f POF IV
|x 0
588 _ _ |a Dataset connected to DataCite
650 _ 7 |a Molecular Neuroscience
|2 Other
650 _ 7 |a Phosphorylation
|2 Other
650 _ 7 |a Protein-Protein Interaction
|2 Other
650 _ 7 |a Signal Transduction
|2 Other
650 _ 7 |a Cellular Neuroscience
|2 Other
650 _ 7 |a Presynaptic Mechanisms of Synaptic Transmission
|2 Other
650 _ 7 |a Protein Phosphorylation
|2 Other
700 1 _ |a Nagy-Herczeg, Domonkos
|b 1
700 1 _ |a Aguilar Perez, Gerard
|0 P:(DE-2719)9002012
|b 2
|u dzne
700 1 _ |a Ranti, Dimitra
|b 3
700 1 _ |a Syropoulou, Vasiliki
|b 4
773 _ _ |a 10.17632/w97nrdvxbc.1
787 0 _ |a Kroon, Cristina et.al.
|d St. Louis : Elsevier, 2025
|i RelatedTo
|0 DZNE-2025-01117
|r
|t Phosphorylation of presynaptic PLPPR3 controls synaptic vesicle release
909 C O |o oai:pub.dzne.de:282939
|p VDB
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 2
|6 P:(DE-2719)9002012
913 1 _ |a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|1 G:(DE-HGF)POF4-350
|0 G:(DE-HGF)POF4-351
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-300
|4 G:(DE-HGF)POF
|v Brain Function
|x 0
914 1 _ |y 2025
920 1 _ |0 I:(DE-2719)1813002
|k AG Milovanovic (Berlin)
|l Molecular Neuroscience
|x 0
980 _ _ |a dataset
980 _ _ |a VDB
980 _ _ |a I:(DE-2719)1813002
980 _ _ |a UNRESTRICTED

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