TY  - JOUR
AU  - Planert, Henrike
AU  - Mittermaier, Franz Xaver
AU  - Grosser, Sabine
AU  - Fidzinski, Pawel
AU  - Schneider, Ulf Christoph
AU  - Radbruch, Helena
AU  - Onken, Julia
AU  - Holtkamp, Martin
AU  - Schmitz, Dietmar
AU  - Alle, Henrik
AU  - Vida, Imre
AU  - Geiger, Jörg Rolf Paul
AU  - Peng, Yangfan
TI  - Electrophysiological classification of human layer 2-3 pyramidal neurons reveals subtype-specific synaptic interactions.
JO  - Nature neuroscience
VL  - 29
IS  - 2
SN  - 1097-6256
CY  - New York, NY
PB  - Nature America
M1  - DZNE-2026-00172
SP  - 455 - 466
PY  - 2026
AB  - Understanding the functional principles of the human brain requires deep insight into its neuronal and network physiology. In superficial layers of temporal cortex, molecular and morphological subtypes of glutamatergic excitatory pyramidal neurons have been described, but subtyping based on electrophysiological parameters has not been performed. The extent to which pyramidal neuron subtypes contribute to the specialization of physiological interactions by forming synaptic subnetworks remains unclear. Here we performed whole-cell patch-clamp recordings of more than 1,400 layer 2-3 (L2-3) pyramidal neurons and 1,400 identified monosynaptic connections in acute slices of human temporal cortex. We extract principles of neuronal and synaptic physiology along with anatomy and functional synaptic connectivity. We also show robust classification of pyramidal neurons into four electrophysiological subtypes, corroborated by differences in morphology and decipher subtype-specific synaptic interactions. Principles of microcircuit organization are found to be conserved at the individual level. Such a fine network structure suggests that the functional diversity of pyramidal neurons translates into differential computations within the L2-3 microcircuit of the human cortex.
KW  - Humans
KW  - Pyramidal Cells: physiology
KW  - Pyramidal Cells: classification
KW  - Pyramidal Cells: cytology
KW  - Synapses: physiology
KW  - Patch-Clamp Techniques
KW  - Temporal Lobe: physiology
KW  - Temporal Lobe: cytology
KW  - Male
KW  - Female
KW  - Adult
KW  - Nerve Net: physiology
KW  - Middle Aged
LB  - PUB:(DE-HGF)16
C6  - pmid:41372679
C2  - pmc:PMC12880919
DO  - DOI:10.1038/s41593-025-02134-7
UR  - https://pub.dzne.de/record/285048
ER  -