Journal Article

DZNE-2026-00172

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Electrophysiological classification of human layer 2-3 pyramidal neurons reveals subtype-specific synaptic interactions.

Planert, H. ; Mittermaier, F. X. ; Grosser, S. ; Fidzinski, P. ; Schneider, U. C. ; Radbruch, H. ; Onken, J. ; Holtkamp, M. ; Schmitz, D.DZNE* ; Alle, H. ; Vida, I. ; Geiger, J. R. P. ; Peng, Y.

2026
Nature America New York, NY

This record in other databases:    

Please use a persistent id in citations: doi:10.1038/s41593-025-02134-7

Abstract: Understanding the functional principles of the human brain requires deep insight into its neuronal and network physiology. In superficial layers of temporal cortex, molecular and morphological subtypes of glutamatergic excitatory pyramidal neurons have been described, but subtyping based on electrophysiological parameters has not been performed. The extent to which pyramidal neuron subtypes contribute to the specialization of physiological interactions by forming synaptic subnetworks remains unclear. Here we performed whole-cell patch-clamp recordings of more than 1,400 layer 2-3 (L2-3) pyramidal neurons and 1,400 identified monosynaptic connections in acute slices of human temporal cortex. We extract principles of neuronal and synaptic physiology along with anatomy and functional synaptic connectivity. We also show robust classification of pyramidal neurons into four electrophysiological subtypes, corroborated by differences in morphology and decipher subtype-specific synaptic interactions. Principles of microcircuit organization are found to be conserved at the individual level. Such a fine network structure suggests that the functional diversity of pyramidal neurons translates into differential computations within the L2-3 microcircuit of the human cortex.

Keyword(s): Humans (MeSH) ; Pyramidal Cells: physiology (MeSH) ; Pyramidal Cells: classification (MeSH) ; Pyramidal Cells: cytology (MeSH) ; Synapses: physiology (MeSH) ; Patch-Clamp Techniques (MeSH) ; Temporal Lobe: physiology (MeSH) ; Temporal Lobe: cytology (MeSH) ; Male (MeSH) ; Female (MeSH) ; Adult (MeSH) ; Nerve Net: physiology (MeSH) ; Middle Aged (MeSH)

---

Contributing Institute(s):

1. Network Dysfunction (AG Schmitz)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2026

---

Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Nature ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 25 ; JCR ; National-Konsortium ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---